Abstract
The autogamous diploid annualMicroseris douglasii of California occurs in many isolated populations. The populations consist of one to many highly inbred biotypes. Morphological variation among populations usually is greater than within populations. In spite of the virtual absence of gene flow even within populations, genetically determined character differences are randomly distributed and associated throughout the range of the species. Recent evidence even suggests introgression of chloroplasts from the relatedM. bigelovii. Offspring families from 25 plants of a very variable population were raised and examined for segregation of morphological and molecular (RAPD) markers. All 25 original plants were completely homozygous for all markers, but each differed from all others at least in some markers. The population consisted of two genetically isolated groups of plants: a distinct inbred line (3 plants) and 22 plants with random associations of a common set of markers and characters, possibly recombinant inbreds from a past hybridization event. One of these 22 plants contained a chloroplast genome found inM. bigelovii, the other 24 plants a chloroplast genome found only inM. douglasii.
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References
Bachmann, K., Battjes, J., 1994: Variability in a predominantly self-fertilizing annual with fragmented distribution,Microseris douglasii (Asteraceae, Lactuceae). — Biol. Zentralblatt113: 69–95.
—, 1984: Differential geographic distribution of spatulate and ponted leaf shapes inMicroseris bigelovii (Asteraceae, Lactuceae). — Beitr. Biol. Pfl.59: 5–14.
—, 1992: Spatulate leaves: a marker gene for the evolution ofMicroseris bigelovii (Asteraceae, Lactuceae). — Beitr. Biol. Pfl.57: 167–179.
-Roelofs, T. F. M., 1995: Phenotypic plasticity and parent/offspring regression of characters in inbred strains ofMicroseris douglasii (Asteraceae, Lactuceae). — Biol. Zentralblatt (in press).
Chambers, K. L., 1955: A biosystematic study of the annual species ofMicroseris. — Contrib. Dudley Herb.4: 207–312.
Feinberg, A. P., Vogelstein, B., 1983: A technique for radiolabeling DNA restriction fragments to high specific activity. — Anal. Biochem.132: 6–13.
Felsenstein, J., 1985: Confidence limits on phylogenies: an approach using the bootstrap. — Evolution39: 783–791.
Jansen, R. K., Palmer, J. D., 1987: Chloroplast DNA from lettuce andBarnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion. — Curr. Genet.11: 553–564.
Mauthe, S., Bachmann, K., Chambers, K. L., Price, H. J., 1984a: Inheritance of heterocarpy inMicroseris douglasii strain C29 (Asteraceae, Lactuceae). — Biol. Zentralblatt103: 495–504.
—, 1984b: Independent responses of two fruit characters to developmental regulation inMicroseris douglasii (Asteraceae, Lactuceae). — Experientia40: 1280–1281.
—, 1985: Genetics of heterocarpy and pappus part numbers inMicroseris douglasii strain C28 (Asteraceae, Lactuceae). — Biol. Zentralblatt104: 1–20.
—, 1985: Inheritance of nuclear 2C DNA content in a cross betweenMicroseris douglasii andM. bigelovii (Asteraceae). — Biol. Zentralblatt104: 269–276.
—, 1986: Patterns of mean nuclear DNA content inMicroseris douglasii (Asteraceae) populations. — Bot. Gaz.147: 496–507.
Rieseberg, L. H., Brunsfeld, S. J., 1992: Molecular evidence and plant introgression. InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 151–176. — New York: Chapman & Hall.
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. — Proc. Natl. Acad. Sci. USA81: 8014–8018.
Swofford, D. L., Olsen, G. J., 1990: Phylogeny reconstruction. — InHillis, D. M., Moritz, C., (Eds): Molecular systematics, pp. 411–501. — Sunderland, Ma.: Sunderland.
Vlot, E. C., Bachmann, K., 1990: Genetics of the proportion of peripheral yellow achenes on the capitula ofMicroseris douglasii strain D37 (Asteraceae, Lactuceae). — Acta Bot. Neerl.39: 229–240.
—, 1991: Genetic and non-genetic variation in the number of pappus parts inMicroseris douglasii strain D37 (Asteraceae, Lactuceae). — Ann. Bot.68: 235–241.
—, 1993: Genetics of the number of achenes and involucral bracts in interstrain hybrid D37 ofMicroseris douglasii (Asteraceae, Lactuceae). — Biol. Zentralblatt112: 7–17.
—, 1992: Genetic and non-genetic factors influencing deviations from five pappus parts in a hybrid betweenMicroseris douglasii andM. bigelovii (Asteraceae, Lactuceae). — Int. J. Pl. Sci.53: 89–97.
Wallace, R. S., Jansen, R. K., 1990: Systematic implications of chloroplast DNA variation in the genusMicroseris (Asteraceae, Lactuceae). — Syst. Bot.15: 606–616.
Welsh, J., McClelland, M., 1990: Fingerprinting genomes using PCR with arbitrary primers. — Nucleic Acids Res.18: 7213–7218.
Wendel, J. F., McD Stewart, J., Rettig, J. H., 1991: Molecular evidence for homoploid reticulate evolution among Australian species ofGossypium. — Evolution45: 694–711.
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V., 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res.18: 6531–6535.
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Roelofs, D., Bachmann, K. Chloroplast and nuclear DNA variation among homozygous plants in a population of the autogamous annualMicroseris douglasii (Asteraceae, Lactuceae). Pl Syst Evol 196, 185–194 (1995). https://doi.org/10.1007/BF00982959
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DOI: https://doi.org/10.1007/BF00982959