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Prooxidant and antioxidant effects of trolox on ferric ion-induced oxidation of erythrocyte membrane lipids

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Abstract

The prooxidant and antioxidant actions of Trolox were examined in anin vitro system measuring ferric ion-induced oxidation of erythrocyte membrane lipids. Trolox was found to produce a concentration-dependent biphasic effect on the ferric ion-stimulated lipid peroxidation, with the mode of action being similar to those produced by reducing-agent antioxidants, such as ascorbic acid and reduced glutathione, and iron chelator, such as desferrioxamine. Phytic acid, a potent iron chelator, could suppress the prooxidant actions of Trolox and desferrioxamine, but not those of ascorbic acid and reduced glutathione. The ability of Trolox to stimulate ferric ion-catalyzed ascorbate oxidation, as similar to the action produced by ethylenediaminetetraacetic acid, indicates the presence of iron-chelating activity. The ensemble of results suggests the possible involvement of iron chelation in the prooxidant action of Trolox in ferric ion-stimulated lipid peroxidation reactions.

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Abbreviations

ASC:

ascorbic acid

BHT:

butylated hydroxytoluene

DESF:

desferrioxamine

EDTA:

ethylenediamine-tetraacetic acid

GSH:

reduced glutathione

PHY:

phytic acid

TBA:

thiobarbituric acid

TBARS:

thiobarbituric acid-reactive substances

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Authors and Affiliations

  1. Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong

    Kam Ming Ko, Pak Kin Yick, Michael K. T. Poon & Siu Po Ip

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  1. Kam Ming Ko
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  2. Pak Kin Yick
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  3. Michael K. T. Poon
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  4. Siu Po Ip
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Ko, K.M., Yick, P.K., Poon, M.K.T. et al. Prooxidant and antioxidant effects of trolox on ferric ion-induced oxidation of erythrocyte membrane lipids. Mol Cell Biochem 141, 65–70 (1994). https://doi.org/10.1007/BF00935592

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  • DOI: https://doi.org/10.1007/BF00935592

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