Abstract
Echinococcus multilocularis protoscolices collected from experimentally infected jirds were incubated for 2 weeks in rat hepatic cell cultures cocultivated with or without feeder cells (BALB/c 3T3 and IAR 20). Scanning and transmission electron microscopy studies were performed during the course of the culture period. Kupffer cells (Kc) were seen adhering to the anterior and posterior ends of the protoscolices. Some protoscolices were fixed to the cell monolayer by a cluster of Kc adhering to the posterior end of the parasite. These cells were phagocytosing the glycocalyx and the electrondense distal end of the microthrix of the protoscolex tegument. An alteration in the superficial tegumental cytoplasm with extensive mitochondrial damage was also noted. The properties expressed by Kc against protoscolices in vitro might also be relevant for proliferation of metacestodes in vivo.
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References
Ali-Khan Z (1978)Echinococcus multilocularis: cell mediated immune response in early and chronic alveolar murine hydatidosis. Exp Parasitol 4:157–165
Araj GF, Matossian RM, Malakian AH (1977) The host response in secondary hydatidosis of mice. II Cell mediated immunity. Z Parasitenkd 52:31–38
Baron RW, Tanner CE (1977)Echinococcus multilocularis in the mouse: the in vitro protoscolicidal activity of peritoneal macrophages. Int J Parasitol 7:489–495
Capron M, Capron A, Torpier G, Bazin H, Bout D, Joseph M (1978) Eosinophil-dependent cytotoxicity in rat schistosomiasis. Involvement of Ig G2a antibody and role of mast cells. Eur J Immunol 8:127–133
Crocker RR (1986) Macrophage parasite interactions in vitro: potential use of extracellular matrix. Parasitol Today 2:148–150
Eckert J, Thompson RCA, Mehlhorn H (1983) Proliferation and metastase formation of larvalEchinococcus multilocularis. I Animal model, macroscopical and histological findings. Z Parasitenkd 69:737–748
Engelkirk PG, Williams JF, Signs MM (1981) Interaction betweenTaenia taeniaeformis and host cells in vitro. Rapid adherence of peritoneal cells to strobilocerci. Int J Parasitol 11:463–474
Fahimi HD (1970) The fine structural localization of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver. J Cell Biol 47:247–262
Frank W (1976) Parasitologie. Eugen Ulmer, Stuttgart
Kakunaga T (1973) A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB/c 3T3. Int J Cancer 12:463–473
Kirkpatrick CJ, Svilenov D (1987) Studies on the adhesion of protoscolices fromEchinococcus multilocularis andE. granulosus to artificial substrates and human endothelial cells in vitro. Parasitol Res 74:61–68
McManus DP, Barrett NJ (1985) Isolation, fractionation and partial characterization of the tegumental surface from protoscolices of the hydatid organismEchinococcus granulosus. Parasitology 90:111–129
Meis JFG, Verhave JP, Jap PHK, Meuwissen JHET (1983) An ultrastructural study on the role of Kupffer cells in the process of infection byPlasmodium berghei sporozoites in rats. Parasitology 86:231–242
Mesnil M, Fraslin JM, Piccoli C, Yamasaki H, Guguen-Guillouzo C (1987) Cell contact but not junctional communication (dye coupling) with biliary epithelial cells is required for hepatocytes to maintain differentiated functions. Exp Cell Res 173:524–533
Morseth DJ (1967) Fine structure of the hydatid cyst and protoscolices ofEchinococcus granulosus. J Parasitol 53:312–325
Rau ME, Tanner CE (1973)Echinococcus multilocularis in the cotton rat. The effect of pre-existing subcutaneous cysts on the development of a subsequent intraperitoneal inoculum of protoscolices. Can J Zool 51:55–59
Rau ME, Tanner CE (1976)Echinococcus multilocularis in the cotton rat: the in vitro protoscolicidal activity of peritoneal cells. Int J Parasitol 6:195–197
Seglen PO (1973) Preparation of rat liver cells. III. Enzymatic requirements for tissue dispersion. Exp Cell Res 82:391–398
Seljelid R (1980) Properties of Kupffer cells. In: Van Furth R (ed) Mononuclear phagocytes: functional aspects. Martinus Nijhoff, The Hague
Smyth JD (1979)Echinococcus granulosus andE. multilocularis: in vitro culture of the strobilar stages from protoscolices. Angew Parasitol 20:137–147
Smyth JD, Barrett NJ (1980) Procedures for testing the viability of human hydatid cysts following surgical removal, especially after chemotherapy. Trans R Soc Trop Med Hyg 74:649–652
Varela-Diaz VM, Coltorti EA (1972) Further evidence of the passage of host immunoglobulins into hydatid cysts. J Parasitol 58:1015–1016
Wardlaw AC, Howard JG (1959) A comparative survey of the phagocytosis of different species of bacteria by Kupffer cells. Br J Exp Pathol 40:113–117
Zachariou Z, Kubelka CF, Rauterberg EW, Krug M, Knierim M, Ruppela A (1986) New properties of Kupffer and endothelial cells isolated from rats infected with the parasiteSchistosoma mansoni. In: Kirn A, Knook DL, Wisse E (eds) Cells of the hepatic sinusoid, vol 1. Kupffer Cell Foundation, RIJSWIJK, The Netherlands, pp 329–334
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Walbaum, S., al Nahhas, S., Gabrion, C. et al. Echinococcus multilocularis: In vitro interactions between protoscolices and Kupffer cells. Parasitol Res 80, 381–387 (1994). https://doi.org/10.1007/BF00932375
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DOI: https://doi.org/10.1007/BF00932375