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ADP-ribosylation and gene expression

  • Part II: Poly(ADP-ribosyl)ation
  • C. Cellular Functions
  • Published:
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Abstract

Gene expression can be defined as the conversion of information existing in a molecule of DNA into a mature RNA or protein product and each step in the process, which requires the concerted action of several macromolecules for completion, may be perturbed by the post-translational modification of specific proteins with ADP-ribose. The participation of poly(ADP-ribose) in the regulation of transcription initiation was examined using cell-free systems for both ribosomal RNA and ribosomal proteins. The presence or absence of poly(ADP-ribose) polymerase did not influence the transcription process. Similarly, under conditions optimal for poly(ADP-ribose) polymerase activity, no change in transcription was observed. A direct contribution of poly(ADP-ribosyl)ation to gene transcription thus could not be detected. In contrast, the addition of 3-aminobenzamide to quiescent hepatoma cells treated with insulin inhibited the stimulation of rRNA synthesis. The high concentrations necessary for this effect suggest that a mono(ADP-riboysl)ation event participates in the cellular action of insulin. A role in the signal transduction pathway leading to activation of rRNA gene expression has been proposed.

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Authors and Affiliations

  1. Division of Cardiovascular Sciences, Department of Physiology, St. Boniface General Hospital Research Centre, University of Manitoba, 351 Tache Avenue, R2H 2A6, Winnipeg, Manitoba, Canada

    Peter Zahradka & Lorraine Yau

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  1. Peter Zahradka
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  2. Lorraine Yau
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Zahradka, P., Yau, L. ADP-ribosylation and gene expression. Mol Cell Biochem 138, 91–98 (1994). https://doi.org/10.1007/BF00928448

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  • DOI: https://doi.org/10.1007/BF00928448

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