Abstract
The activity and kinetics of acid-stable protease inhibitor (ASPI) were investigated in the chroric phase of carrageenin-induced inflammation in rats. The ASPI activity was 19.6+3.1 units/ml in the plasma and 15.4+2.1 units/ml in the inflammatory exudate. The plasma value was significantly higher than that of the control (11.6+1.3 units/ml). A kinetics study was performed using purified and radiolabeled rat plasma ASPI, whose NH2-terminal amino acid sequence was Ala-Val-Leu-Pro-Gln-Glu-Asn-Glu-Gly-X-Gly-Ser-Glu-Pro-Leu-Ile-Thr-Gly-Thr-Leu-Lys-Lys-Glu-Asp-Ser-Asn-Gln-Leu-Lys-Tyr-Ser-Glu-Gly-Pro. The half-life of the distributive phase was 4.3+0.4 min and that of the postdistributive phase (biological half-life) was 42.2+9.2 min in inflammation. There was no significant difference compared with the values in the control (3.9+0.4 min and 40.7+6.5 min, respectively). It appeared that the increase in ASPI in inflammation was not due to prolonged excretion of the inhibitor but to an increased production of it, and ASPI was rapidly distributed to the fluids and tissues.
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Balduyck, M., C. Mizon, H. Ioutfi, C. Richet, P. Roussel, andJ. Mizon. 1986. The major human urinary trypsin inhibitor is a proteoglycan.Eur. J. Biochem. 158:417–422.
Gosset, D., C. Mizon, P. Savinel, M. Balduyck, B. Boniface, P. Y. Hatron, J. Mizon, andB. Devulder. 1988. Clinical value of the determination of urinary antitrypsin activity.Presse Med. 17:329–332.
Toki, N., andH. Sumi. 1982. Urinary trypsin inhibitor and urokinase activities in renal diseases.Acta Haematol. 67:109–113.
Gebhard, W., andK. Hochstrasser. 1986. Inter-α-trypsin inhibitor and its close relatives.In Proteinase Inhibitors. A. J. Barrett, and G. Salvesen, editors. Eisevier Science Publishers, Amsterdam. 389–401.
Shikimi, T., andM. Handa. 1986. Inhibitory effect of human urinary trypsin inhibitor (urinastatin) on lysosomal thiol proteinases.Jpn. J. Pharmacol. 42:571–574.
Kikuchi, H., S. Tanaka, andO. Matsuo. 1987. Plasminogen activator on synovial fluid from patients with rheumatoid arthritis.J. Rheumatol. 14:439–445.
Matsuo, O., S. Tanaka, andH. Kikuchi. 1988. Effect of urinary trypsin inhibitor on osteoarthritis.Thromb. Res. 52:237–245.
Maruyama, M., H. Sumi, K. Akazawa, andH. Mihara. 1984. Marked increase of plasma acid stable/soluble trypsin inhibitor in hemodialysis patients.Clin. Chim. Acta 138:205–213.
Akazawa, K., H. Sumi, M. Maruyama, andH. Mihara. 1983. Acid stable protease inhibitor in ascites of ovarian carcinoma.Clin. Chim. Acta 131:87–99.
Yamamoto, T., H. Sumi, M. Maruyama, H. Mizumoto, R. Ikeda, H. Yoshihara, andH. Mihara. 1986. Acid stable trypsin inhibitor in bile.Clin. Chim. Acta 158:91–98.
Okumichi, T., M. Nishiki, S. Takasugi, N. Toki, andH. Ezaki. 1984. Isolation of urinary trypsin inhibitor-like inhibitor from human lung cancer tissue.Cancer Res. 44:2011–2015.
Sugiki, M., H. Sumi, M. Maruyama, E. Yoshida, andH. Mihara. 1989. Clearance and distribution of acid-stable trypsin inhibitor (ASTI).Enzyme 42:31–38.
Hochstrasser, K., J. Niebel, H. Feuth, andK. Lempart. 1977. Über Abbauprodukte des Inter-alpha-Trypsininhibitors im Serum. I. Der Inter-alpha-Trypsininhibitor als Prekursor des säurestabilen Serum-Trypsin-Plasmin-Inhibitors.Klin. Wochenschr. 55:337–342.
Franck, C., andJ. Z. Pedersen. 1983. Trypsin-inhibitory activities of acid-stable fragments of the inter-alpha-trypsin inhibitor in inflammatory and uraemic conditions.Scand. J. Lab. Invest. 43:151–155.
Sumi, H., E. Yoshida, H. Hamada, andH. Mihara. 1988. Acid-stable trypsin-plasmin inhibitors formed enzymatically from plasma precursor protein.Biochim. Biophys. Acta 966:1–11.
Salier, J. P., R. Sesboue, K. Hochstrasser, O. Schonberger, andJ. P. Martin. 1983. Isolation and characterization of an inter-α-trypsin inhibitor-IgG complex from human serum.Biochim. Biophys. Acta 742:206–214.
McKeehan, W. L., Y. Sakagami, H. Hoshi, andK. A. McKeehan. 1986. Two apparent human endothelial cell growth factors from human hepatoma cells are tumor-associated proteinase inhibitors.J. Biol. Chem. 261:5378–5383.
Tsurufuji, S. 1979. How to assess inflammation in animals.Drugs Exp. Clin. Res. 5:79–84.
Maruyama, M., T. Yamamoto, H. Sumi, H. Tsushima, H. Mihara, andN. Minamino. 1986. Purification and amino acid analysis of plasma acid stable trypsin inhibitor.Enzyme 35:225–231.
Sumi, H., M. Maruyama, H. Mihara, S. Maehara, andN. Toki. 1981. Human urinary trypsin inhibitors purified by affinity chromatography on trypsin-Sepharose.Acta Haematol. Jpn. 44:146–153.
Vesterbero, O., andH. Svensson. 1966. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients.Acta Chem. Scand. 20:820–827.
Noda, M., S. Shimizu, T. Tanabe, T. Takai, T. Kayano, T. Ikeda, H. Takahashi, H. Nakayama, Y. Kanaoka, N. Minamino, K. Kangawa, H. Matsuo, A. Raftery, T. Hirose, S. Inayama, H. Hayashida, T. Miyata, andS. Numa. 1984. Primary structure of electrophorus electricus sodium channel deduced from cDNA sequence.Nature 312:121–127.
Gibaldi, M., andD. Perrier. 1975. Pharmacokinetics.In Drugs and the Pharmaceutical Sciences, Vol. 1. J. Swarbrick, editor. Marcel Dekker, New York. 45–96.
Hochstrasser, K., G. Bretzel, H. Feuth, W. Hilla, andK. Lempart. 1976. Inter-α-trypsin inhibitor as precursor of the acid-stable proteinase inhibitors on human serum and urine.Hoppe-Seyler's Z. Physiol. Chem. 357:153–162.
Reisinger, P., K. Hochstkasser, G. J. Albrecht, K. Lempart, andJ. P. Salier. 1985. Human inter-α-trypsin inhibitor: Localization of the Kunitz-type domains in the N-terminal part of the molecule and their release by a trypsin-like proteinase.Biol. Chem. Hoppe-Seyler 366:479–483.
Pratt, C. W., M. W. Swaim, andS. V. Pizzo. 1989. Inflammatory cells degrade inter-α inhibitor to liberate urinary proteinase inhibitors.J. Leukocyte Biol. 45:1–9.
Yoshida, E., H. Sumi, M. Maruyama, H. Tsushima, Y. Matsuoka, M. Sugiki, andH. Mihara. 1989. Distribution of acid stable trypsin inhibitor immunoreactivity in normal and malignant human tissues.Cancer 64:860–869.
Kosugi, T., I. Takagi, O. Matsuo, H. Mihara, andO. Nishikaze. 1981. Antiplasmin activity in carrageenin-induced inflammation of rats.Int. J. Tissue React. III:173–176.
Nakagawa, H., M. Isaji, M. Hayashi, andS. Tsurufuji. 1981. Selective inhibition of collagen breakdown by proteinase inhibitors in granulation tissue in rats.J. Biochem. 89:1081–1090.
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Sugiki, M., Maruyama, M., Yoshida, E. et al. Acid-stable protease inhibitor in chronic phase of carrageenin-induced inflammation in rats. Inflammation 15, 281–289 (1991). https://doi.org/10.1007/BF00917313
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DOI: https://doi.org/10.1007/BF00917313