Skip to main content
SpringerLink
Log in

Polymerase chain reaction amplification of messages for growth factors in cells from human bronchoalveolar lavage fluids

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Genetic messages for polypeptide growth factors were assessed in human alveolar macrophages, obtained by bronchoalveolar lavage (BAL) from normal subjects (N=3) and from patients with pneumonia (N=3), pulmonary lymphoma (N =3), and idiopathic pulmonary fibrosis (N=3). Complementary DNAs (cDNAs) were prepared by reverse transcription of the RNA extracted from alveolar macrophages before and afterculture on a plastic surface. The cDNAs encoding 10 different growth factors were amplified for electrophoretic analysis by polymerase chain reaction with a pair of 3′ and 5′ primers specific for each factor. Alveolar macrophages from all normal subjects and patients expressed the messages for interleukin1β and transforming growth factor-β. Alveolar macrophages from some normal subjects also contained message for insulin-like growth factor-1. Alveolar macrophages from six of nine patients with lung diseases also expressed messages for one or more additional growth factors, including epidermal growth factor, transforming growth factor-α, interleukin-1α, and platelet-derived growth factor. The polymerase chain reaction technique thus permits determination of the profile of growth factors contributed to pulmonary reactions by alveolar macrophages, which may be important in pulmonary healing and fibrosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sporn, M. B., andE. D. Harris. 1987. Proliferative diseases.Am. J. Med. 70:1231–1235.

    Google Scholar 

  2. Schilling, J. A., B. V. Favata, andM. D. Radakovich. 1953. Studies of fibroplasia in wound healing.Surg. Gynecol. Obstet. 96:143–149.

    Google Scholar 

  3. Wahl, S. M., L. M. Wahl, andJ. B. McCarthy. 1978. Lymphocyte-mediated activation of fibroblast proliferation and collagen production.J. Immunol. 121:942–946.

    Google Scholar 

  4. Banda, M. J., D. R. Knighton, T. K. Hunt, andZ. Werb. 1982. Isolation of a nonmitogenic angiogenesis factor from wound fluid.Proc. Natl. Acad. Sci. U.S.A. 79:7773–7777.

    Google Scholar 

  5. Hibbs, M. S., A. E. Postlethwaite, C. L. Mainardi, J. M. Seyer, andA. H. Kang. 1983. Alterations in collagen production in mixed mononuclear leukocyte-fibroblast cultures.J. Exp. Med. 157:47–59.

    Google Scholar 

  6. Turck, C. W., J. G. Dohlman, andE. J. Goetzl, 1987. Immunological mediators of wound healing and fibrosis.J. Cell. Physiol. (Suppl.) 5:89–93.

    Google Scholar 

  7. Auron, P. E., A. C. Webb, andL. J. Rosenwasser. 1984. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.Proc. Natl. Acad. Sci. U.S.A. 81:7907–7911.

    Google Scholar 

  8. Derynck, R., A. B. Roberts, M. E. Winckler, E. Y. Chen, andD. V. Goeddel. 1984. Human transforming growth factor-alpha precursor structure and expression inE. coli. Cell 38:287–297.

    Google Scholar 

  9. Baird, A., P. Mormede, andP. Boehlen. 1985. Immunoreactive fibroblast growth factor in cells of peritoneal exudate suggests its identity with macrophage-derived growth factor.Biochem. Biophys. Res. Commun. 126:358–364.

    Google Scholar 

  10. Derynck, R., J. A. Jarrett, andE. Y. Chen. 1985. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.Nature 316:701–705.

    Google Scholar 

  11. Abraham, J. A., J. L. Whang, andA. Tumolo. 1986. Nucleotide sequence of a bovine clone encoding and angiogenic protein, basic FGF.Science 233:545–548.

    Google Scholar 

  12. Bell, G. J., N. M. Fong, andM. M. Stempien. 1986. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organisation.Nucleic Acids Res. 14:8427–8446.

    Google Scholar 

  13. Betsholtz, C., A. Johnsson, andC. H. Heldin. 1986. cDNA sequence and chromosomal localisation of human platelet-derived growth factor A-chain and its expression in tumor cell lines.Nature 320:695–699.

    Google Scholar 

  14. Furutani, Y., M. Notake, andT. Fukui. 1986. Complete nucleotide sequence of the gene for human interleukin 1-alpha.Nucleic Acids Res. 14:3167–3179.

    Google Scholar 

  15. Jaye, M., R. Howk, andW. Burgess. 1986. Human endothelial cell growth factor: Cloning, nucleotide sequence, and chromosome localisation.Science 233:541–545.

    Google Scholar 

  16. Le Bouc, Y. L., D. Dreyer, F. Jaeger, M. Binoux, andP. Sondermeyer. 1986. Complete characterisation of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108–112.

    Google Scholar 

  17. Rao, C. D., H. Igarashi, I.-M. Chiu, K. C. Robbins, andS. A. Aaronson. 1986. Structure and sequence of the human c-sis/platelet-derived growth factor 2 (sis/pdgf2) transcriptional unit.Proc. Natl. Acad. Sci. U.S.A. 83:2392–2396.

    Google Scholar 

  18. Dinarello, C. A. 1988. Biology of interleukin-1.FASEB J. 2:108–115.

    Google Scholar 

  19. Turck, C. W., J. W. Tom, P. W. Kennedy, andE. J. Goetzl. 1988. Isolation and partial characterization of a fibroblast-activating factor generated by U-937 human monocytic leukocytes.J. Immunol. 141:1225–1230.

    Google Scholar 

  20. Crystal, R. G., P. B. Bitterman, S. I. Rennard, A. J. Hance, andB. A. Keogh. 1984. Interstitial lung disease of unknown cause: disorders characterized by chronic inflammation of the lower respiratory tract.N. Engl. J. Med. 310:154–166.

    Google Scholar 

  21. Mornex, J. F., Y. Martinet, andK. Yamauchi. 1986. Spontaneous expression of thec-sis gene and release of a platelet-derived growth factor-like molecule by human alveolar macrophages.J. Clin. Invest. 78:61–66.

    Google Scholar 

  22. Mullis, K. B., andF. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.Methods Enzymol. 155:335–368.

    Google Scholar 

  23. Golden, J. 1986. Bronchoalveolar lavage.West. J. Med. 145:362–368.

    Google Scholar 

  24. Erba, H. P., R. Eddy, T. Shows, L. Kedes, andP. Gunning. 1988. Structure, chromosome location, and expression of the human gamma-actin gene: Differential evolution, location, and expression of the cytoskeletal beta and gamma-actin genes.Mol. Cell Biol. 8:1775–1789.

    Google Scholar 

  25. Rappolee, D. A., D. Mark, M. J. Banda, andZ. Werb. 1988. Wound macrophages express TGF-alpha and other growth factors in vivo: Analysis by mRNA phenotyping.Science 211:708–712.

    Google Scholar 

  26. Rom, W. N., P. Basset, G. A. Fells, T. Nukiwa, B. C. Trapnell, andR. G. Crystal. 1988. Alveolar macrophages release an insulin-like growth factor I-type molecule.J. Clin. Invest. 82:1685–1693.

    Google Scholar 

  27. Dike, L. E., andS. R. Farmer. 1988. Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.Proc. Natl. Acad. Sci. U.S.A. 85:6792–6796.

    Google Scholar 

  28. Terracio, L., L. Ronnstrand, andA. Tigstrom. 1988. Induction of platelet-derived growth factor receptor expression in smooth muscle cells and fibroblasts upon tissue culturing.J. Cell. Biol. 107:1947–1957.

    Google Scholar 

  29. Fava, R., N. Olsen, J. Keski-oja, H. Moses, andT. Pincus. 1989. Active and latent forms of transforming growth factor-beta activity in synovial effusions.J. Exp. Med. 169:291–296.

    Google Scholar 

  30. Singh, J. P., andP. D. Bonin. 1988. Purification and biochemical properties of a human monocyte-derived growth factor.Proc. Natl. Acad. Sci. U.S.A. 85:6374–6378.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Medicine and Microbiology-Immunology and the Howard Hughes Medical Institute, University of California Medical Center, San Francisco, California

    Christine H. Adolff, Jeffrey A. Golden, Phillip W. Kennedy, Edward J. Goetzl & Christoph W. Turck

Authors
  1. Christine H. Adolff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey A. Golden
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Phillip W. Kennedy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Edward J. Goetzl
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christoph W. Turck
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adolff, C.H., Golden, J.A., Kennedy, P.W. et al. Polymerase chain reaction amplification of messages for growth factors in cells from human bronchoalveolar lavage fluids. Inflammation 15, 259–268 (1991). https://doi.org/10.1007/BF00917311

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00917311

Keywords

Search

Navigation