Abstract
Contrary to resting cells, neutrophils stimulated with concanavalin A resist inhibition by bifunctionalN-hydroxysuccinimide esters. Con A prestimulated, cross-linker-treated cells released superoxide upon restimulation with PMA but did not respond to chemotactic peptides. Although rates of PMA-elicited NADPH oxidase activity were lowered by the treatment, the activation parameters, namely lag times of the reaction, were not altered. The protection by Con A against blockade by cross-linkers developed concomitantly to the activation of NADPH oxidase and indicated redistribution of cross-linker-susceptible cellular components responsible for activation with PMA. The identity of these components is discussed.
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Aviram, I., Sharabani, M. Activation-dependent redistribution of cellular components alters susceptibility of human neutrophils to cross-linking agents. Inflammation 10, 233–242 (1986). https://doi.org/10.1007/BF00916119
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DOI: https://doi.org/10.1007/BF00916119