Abstract
Hybridization studies using specific cDNA have been used to determine the mRNA levels for rat apolipoproteins AI, AII, AIV, and E in extracts of rat liver and intestine. The ratios of intestinal mRNA/liver mRNA for apolipoprotein AI (apo AI), apo AIV, and apo E were 1.3, 1.7, and 0.1, respectively. Apo AII mRNA was detected in the liver but not in the intestine. The mRNA levels for apo AII and apo AIV in rat liver decreased during inflammation to minimums of 40% and 25% of normal, respectively. The mRNA levels for apo AIV in the intestine, apo E in the liver and for apo AI in both the liver and intestine did not change significantly during inflammation. The time course for the decrease in the hepatic mRNA levels for apo AIV was similar to those previously observed for the negative acute-phase proteins albumin and transthyretin. The serum levels for apo AIV were not affected by inflammation.
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Schreiber, G., A. R. Aldred, T. Thomas, H. E. Birch, P. W. Dickson, T. Guofen, P. C. Heinrich, W. Northemann, G. J. Howlett, F. A. de Jong, andA. Mitchell. 1986. Levels of messenger ribonucleic acids for plasma proteins in rat liver during acute experimental inflammation.Inflammation 10:59–66.
Dickson, P. W., G. J. Howlett, andG. Schreiber. 1985. Rat transthyretin (prealbumin): Molecular cloning, nucleotide sequence, and gene expression in liver and brain.J. Biol. Chem. 260:8214–8219.
Ricca, G. A., R. W. Hamilton, J. W. McLean, A. Conn, J. E. Kalinyak, andJ. M. Taylor. 1981. Ratα 1-acid glycoprotein mRNA.J. Biol. Chem. 256:10362–10368.
Birke, G., L. A. Carlson, andS. O. Liljedahl. 1965. Lipid metabolism and trauma. III. Plasma lipids and lipoproteins in burns.Acta Med. Scand. 178:337–350.
Coombes, E. J., P. G. Shakespeare, andG. F. Batstone. 1980. Lipoprotein changes after burns in man.J. Trauma 20:971–975.
Wolfram, G., J. Eckart, andN. Zoller. 1980. Disturbances of lipoprotein and fatty acid metabolism in patients with heavy injuriesKlin. Wochenschr. 58:1327–1337.
Kerttula, Y., M. Vaara, andL. Pyhala. 1982. Effect of bacterial lipopolysaccharide on serum high density lipoprotein cholesterol in rabbits.Atherosclerosis 52:123–126.
Hoffman, J. S., andE. P. Benditt. 1982. Changes in high-density-lipoprotein content following endotoxin administration in the mouse: Formation of serum amyloid protein-rich subfractions.J. Biol. Chem. 257:10510–10517.
Cabana, V. G., H. Gewurz, andJ. N. Siegal. 1983. Inflammation-induced changes in rabbit CRP and plasma lipoproteins.J. Immunol. 130:1736–1742.
Parks, J. S., andL. L. Rudel. 1985. Alteration of high density lipoprotein subfraction distribution with induction of serum anyloid A protein (SAA) in the non human primate,J. Lipid Res. 26:82–91.
Schreiber, G., G. J. Howlett, M. Nagashima, A. Millership, H. Martin, J. Urban, andL. Kotler. 1982. The acute-phase response of plasma protein synthesis during experimental inflammation.J. Biol. Chem. 257:10271–10277.
Catanzaro, D. F., N. Mesterovic, andB. J. Morris. 1985. Studies of the regulation of mouse renin genes by measurement of renin mRNA.Endocrinology 117:872–878.
de Jong, F. A., G. Howlett, A. R. Aldred, N. Fidge andG. Schreiber. 1984. Synthesis of rat apoliproprotein E byEscherichia Coli infected with recombinant bacteriophage.Biochem. Biophys. Res. Commun. 119:657–662.
Morris, G. M., The isolation and characterization of human apolipoprotein cDNA clones, MSc Thesis. University of Melbourne.
Poncin, J. E., J. A. Martial, andJ. E. Gielen. 1984. Cloning and structure analysis of the rat apolipoprotein A-I cDNA.Eur. J. Biochem. 140:493–498.
Boguski, M. S., N. Elshourbagy, J. M. Taylor, andJ. I. Gordon. 1984. Rat apolipoprotein AIV contains 13 tandem repetitions of a 22 amino acid segment with amphipathic helical potential.Proc. Natl. Acad. Sci. U.S.A. 81:5021–5025.
Herbert, P. N., H. G. Windmueller, T. P. Bersot, andR. S. Shulman. 1974. Characterisation of the rat apolipoproteins. The low-molecular-weight proteins of rat plasma high density lipoproteins.J. Biol. Chem. 249:5718–5724.
Nott, T. J., L. M. Priestley, M. Urdea, andJ. Scott. 1984. Isolation and characterisation of a cDNA clone encoding the precursor for human apolipoprotein AII.Biochem. Biophys. Res. Commun. 120:734–740.
Maniatis, T., E. F. Fritsch, andJ. Sambrook. 1982. Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 86–95.
Chirgwin, J. M., A. E. Przybyla, R. J. MacDonald, andW. J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.Biochemistry 18:5294–5299.
Thomas, P.A. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proc. Natl. Acad. Sci. U.S.A. 77:5201–5205.
Fidge, N. 1980. The redistribution and metabolism of iodinated apolipoprotein A-IV in rats.Biochim. Biophys. Acta 619:129–141.
Gordon, J. I., D. P. Smith, D. H. Alpers, andA. W. Strauss. 1982. Cloning of a complementary deoxyribonucleic acid encoding a portion of rat intestinal preapolipoprotein AIV messenger ribonucleic acid.Biochemistry 21:5424–5431.
Wu, A. L. andH. G. Windmueller. 1979. Relative contributions by liver and intestine to individual plasma apolipoproteins in the rat.J. Biol. Chem. 254:7316–7322.
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Guo-Fen, T., De Jong, F., Apostolopoulos, J. et al. Effect of acute inflammation on rat apolipoprotein mRNA levels. Inflammation 11, 241–251 (1987). https://doi.org/10.1007/BF00916024
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DOI: https://doi.org/10.1007/BF00916024