Summary
The occurrence of vacuoles in cells of contractile tissues and especially in media cells of resistance vessels has been known for quite some time. Recently, it has been widely accepted that these vacuoles, characteristically lined by a double membrane, result from herniation of one vascular smooth muscle cell into the other as a result of vasoconstriction. In our electronmicroscopic investigations we found double membrane-bounded vacuoles not only in kidney resistance vessels of rats and mice under conditions of vasoconstriction, but also in control animals and animals with maximal renal vasodilation. Part of our observations are compatible with the assumption that such vacuoles arise from a damage of club-shaped, musculo-muscular contacts due to shape changes of media cells during maximal vasoconstriction or vasodilation. However, serial thin sectioning revealed that some of the cytoplasmic vacuoles have no connections with neighbouring cells. This finding and various parallels to the generation of autophagic vacuoles indicate that the so-called herniations may also represent demarcations of large cytoplasmic areas within an individual cell. Irrespective of the origin of these vacuoles, their contents show different stages of deterioration. At later stages, the vacuoles appear to be adjacent, with only one membrane, to the extracellular space, into which they are believed to discharge finally. Cytoplasmic vacuolization has not only been observed in smooth muscle cells, but also in juxtaglomerular epithelioid cells of the afferent arteriole. Here the vacuoles - besides other organelles - also contain secretory granules; it is therefore proposed that autophagic phenomena with final extrusion of cytoplasmic material may be involved in the programmed down-regulation of the granular renin store following inhibition of renin synthesis and secretion.
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References
Arstila AU, Trump BF (1968) Studies on cellular autophagocytosis. The formation of autophagic vacuoles in the liver after glucagon administration. Am J Pathol 53:687–733
Cain H, Kraus B (1971) Funktions- und Formwandel der Gefäßwandzellen des juxtaglomerulären Apparates der Niere beim Kaninchen. Virchows Arch Abt B Zellpath 7:160–173
Cantin M, Araujo-Nascimento M-de-F, Benchimol S, Desormeaux J (1977) Metaplasia of smooth muscle cells into juxtaglomerular cells in the juxtaglomerular apparatus. Arteries and arterioles of the ischemic (endocrine) kidney. Am J Pathol 87:581–602
Esterly JA, Glagov S (1963) Altered permeability of the renal artery of the hypertensive rat: an electron microscopic study. Am J Pathol 43:619–638
Forssmann WG, Ito S, Weihe E, Aoki A, Dym M, Fawcett DW (1977) An improved perfusion fixation method for the testis. Anat Rec 188:307–314
Gardner DL, Matthews MA (1969) Ultrastructure of the wall of small arteries in early experimental rat hypertension. J Pathol Bacteriol 97:51–62
Goldby FS, Beilin LJ (1972) How an acute rise in arterial pressure damages arterioles. Electron microscopic changes during angiotensin infusion. Cardiovasc Res 6:569–584
Hartman AF (1924) The general physiology and experimental pathology of the suprarenal glands. In: Barker LF (ed) Endocrinology and Metabolism, vol 2; D. Appleton and Company, New York London: pp 101–125
Joris I, Majno G (1977) Cell-to-cell herniae in the arterial wall. I. The pathogenesis of vacuoles in the normal media. Am J Pathol 87:375–398
Joris I, Majno G (1981) Medial changes in arterial spasm induced by L-norepinephrine. Am J Pathol 105:212–222
Lee RMKW, Garfield RE, Forrest JB, Daniel EE (1984) Smooth muscle cell herniation in the contracted arterial wall of spontaneously hypertensive and normotensive rats. Acta Anat 119:65–72
Pfeiffer U (1971) Probleme der cellulären Autophagie. Springer, Berlin Heidelberg New York
Pfeiffer U (1981) Morphological aspects of intracellular protein-degradation: Autophagy. Acta Biol Med Germ 40:1619–1624
Rhodin JAG (1967) The ultrastructure of mammalian arterioles and precapillary sphincters. J Ultrastruct Res 18:181–223
Salgado ED (1970) Medial aortic lesions in rats with metacorticoid hypertension. Am J Pathol 58:305–327
Schwertschlag U, Hackenthal E, Hackenthal R, Rohs GH (1978) The effects of calcium and calcium ionophores (X 537 A and A 23187) on renin release in the isolated perfused rat kidney. Clin Sci Mol Med 55:1635–1665
Taugner R, Kirchheim H, Forssmann WG (1984) Myoendothelial contacts in glomerular arterioles and in renal interlobular arteries of rat, mouse and Tupaia belangeri. Cell Tissue Res 235:319–325
Taugner R, Rosivall L, Bührle CP, Gröschel-Stewart U (1987) Myosin content and vasoconstrictive ability of the proximal and distal (renin-positive) segments of the preglomerular arteriole. Cell Tissue Res 248:579–588
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These studies were supported by the German Research Foundation within the “Forschergruppe Niere, Heidelberg”
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Metz, R., Nobiling, R., Harlacher, M. et al. Cytoplasmic sequestration phenomena in smooth muscle cells of kidney resistance vessels and epithelioid cells of the juxtaglomerular apparatus. Vichows Archiv A Pathol Anat 412, 583–589 (1988). https://doi.org/10.1007/BF00844294
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DOI: https://doi.org/10.1007/BF00844294