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Low molecular weight peptides controlling transcription are present in the calf thymus chromatin structure

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Abstract

A calf thymus peptide fraction controlling DNA and chromatin template has been purified by DNA-cellulose and Dowex 50 WX2 chromatography and its amino acid composition determined. The active peptide fraction can be extracted in high pH buffer from calf thymus native chromatin previously deproteinized by chloroform-isoamylalcohol and phenol. These data demonstrate that the thymic peptide(s) is (are) a chromatin protein constituent strongly linked to DNA. The specificity in association of the peptide(s) to DNA has also been considered.

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Authors and Affiliations

  1. Institute of General Physiology, University of Camerino, Italy

    G. L. Gianfranceschi, L. Guglielmi & D. Amici

  2. Institute of Biological Chemistry, University of Rome, Italy

    F. Bossa, D. Barra & R. Petruzzelli

  3. Laboratory of Applied Biochemistry, University of Camerino, Italy

    F. Bossa, D. Barra & R. Petruzzelli

Authors
  1. G. L. Gianfranceschi
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  2. L. Guglielmi
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  3. D. Amici
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  4. F. Bossa
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  5. D. Barra
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  6. R. Petruzzelli
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Gianfranceschi, G.L., Guglielmi, L., Amici, D. et al. Low molecular weight peptides controlling transcription are present in the calf thymus chromatin structure. Mol Biol Rep 3, 429–436 (1977). https://doi.org/10.1007/BF00808384

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  • DOI: https://doi.org/10.1007/BF00808384

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