Abstract
The principles governing production and the effect on lymphocytes of the mitogenic factor (MF), secreted by human lymphocytes in vitro under the influence of phytohemagglutinin (PHA) and described previously by the author, were investigated. PHA was inactivated by anti-PHA antiserum and immunosorbents. Lymphocytes stimulated by PHA secrete MF in the G1-period and at the beginning of the S-period of the cell cycle. During cultivation of the cells in a protein-free medium DNA and protein synthesis was sharply inhibited but MF production was 1.6 times higher than in media containing serum. The kinetics of the reaction of lymphocytes to MF is described: DNA synthesis (reflected in thymidine-H3 incorporation) began on the 5th–6th day and reached an maximum on the 6th–7th day.
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Voitenok, N.N. Some properties of a phytohemagglutinin-induced lymphocyte mitogenic factor. Bull Exp Biol Med 80, 1079–1082 (1975). https://doi.org/10.1007/BF00799897
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DOI: https://doi.org/10.1007/BF00799897