Abstract
A method of separating rosette-forming cells on columns with an immunosorbent based on Sephadex G-25 and G-75 is described and has been tested. The protein antigen (bovine serum albumin — BSA) is attached by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%.
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Fuks, B.B., Khazanova, I.V. & Yurin, V.L. Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75. Bull Exp Biol Med 78, 792–794 (1974). https://doi.org/10.1007/BF00799322
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DOI: https://doi.org/10.1007/BF00799322