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Origin of somatic embryos from repetitively embryogenic cultures of walnut (Juglans regia L.): Implications forAgrobacterium-mediated transformation

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Abstract

Early stages of somatic embryo development from embryogenic cultures ofJuglans regia (Persian or English walnut) are described. Histological examination reveals that secondary somatic embryos arise from cotyledons and hypocotyls of primary embryos cultured in the dark. The embryos originate by transverse to oblique divisions of surface cells. Single-cell origin of the secondary embryos confirms the potential of the repetitive embryogenesis system forAgrobacterium-mediated transformation and regeneration of non-chimeric, transgenic walnut plants.

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Authors and Affiliations

  1. Department of Pomology, University of California, 95616, Davis, CA, USA

    Vito S. Polito & Katherine Pinney

  2. USDA/ARS, Department of Pomology, University of California, 95616, Davis, CA, USA

    Gale McGranahan & Charles Leslie

Authors
  1. Vito S. Polito
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  2. Gale McGranahan
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  3. Katherine Pinney
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  4. Charles Leslie
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Communicated by G. C. Phillips

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Polito, V.S., McGranahan, G., Pinney, K. et al. Origin of somatic embryos from repetitively embryogenic cultures of walnut (Juglans regia L.): Implications forAgrobacterium-mediated transformation. Plant Cell Reports 8, 219–221 (1989). https://doi.org/10.1007/BF00778537

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  • DOI: https://doi.org/10.1007/BF00778537

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