Abstract
Newly-synthesized cytoplasmic non-mitochondrial DNA was isolated from wheat embryos which had been germinated in the presence of [14C]-thymidine for a time period not long enough to trigger the first post-dormant round of the nuclear DNA replication. This extrachromosomal DNA fraction consisted of linear single-stranded polydeoxyribonucleotide chains, corresponding in size to approximately 1.1×106 daltons, and amounted to about 0.5% of the total cellular DNA content. It is suggested that the appearance of the newly-synthesized polydeoxyribonucleotide chains in the cytoplasm may be a physiological signal for the initiation of the nuclear DNA replication in germinating wheat embryo cells.
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Kraszewska, E., Buchowicz, J. Isolation of a single-stranded extrachromosomal DNA from germinating wheat embryos. Mol Biol Rep 6, 213–217 (1980). https://doi.org/10.1007/BF00777527
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DOI: https://doi.org/10.1007/BF00777527