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Interaction of polyribosomal components and polyribonucleotides with microtubule proteins

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Abstract

To demonstrate the affinity of RNA-containing polyribosomal components (isolated from L5178y cells) to microtubules, microtubule protein was attached to an insoluble matrix. In contrast to ribosomes, poly(A) (+) mRNA and poly(A)-RNP were found to bind to the matrix. Using synthetic polyribonucleotides, no significant differences in the binding properties of single- and double stranded polymers of different base composition to microtubule protein were observed. However, binding is dependent on the size of the polymer; a minimal chain length of 12 nucleotide units is required.

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Universität, Duesbergweg, 6500, Mainz, West Germany

    H. C. Schröder, A. Bernd, R. K. Zahn & W. E. G. Müller

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  1. H. C. Schröder
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  2. A. Bernd
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  3. R. K. Zahn
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  4. W. E. G. Müller
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Schröder, H.C., Bernd, A., Zahn, R.K. et al. Interaction of polyribosomal components and polyribonucleotides with microtubule proteins. Mol Biol Rep 8, 233–237 (1982). https://doi.org/10.1007/BF00776585

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  • DOI: https://doi.org/10.1007/BF00776585

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