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Preparative density gradient centrifugation of RNA and DNA in cesium sulfateurea mixture

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Abstract

A new procedure is described for a large scale separation and purification of unfixed DNA and RNA from a mixture of partially extracted nucleic acids and lysates of subcellular fractions by centrifugation to equilibrium in cesium sulfate-urea mixture. Optimum conditions are described for the separation and quantative recovery of both RNA and DNA in a pure form. The procedure allows determination of peak buoyant densities of 4–5s RNA, 7–11s mRNA and total cytoplasmic RNA. The procedure also allows fractionation of small molecular weight classes of cytoplasmic RNAs from the 18s and 28s rRNAs.

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Authors and Affiliations

  1. Unité de Biologie du Dévéloppement, l'Institut Suisse de Recherches Expérimentales sur le Cancer, 1066, Epalinges, Switzerland

    Sohan P. Modak & Monique Chappuis

  2. Département de Biologie Moléculaire, l'Institut Suisse de Recherches Expérimentales sur le Cancer, 1066, Epalinges, Switzerland

    Sohan P. Modak & Monique Chappuis

  3. Service de Biochimie de la Différenciation, Institut de Biologie Moléculaire du CNRS, Faculté de Sciences, Université de Paris VII, 2 place Jussieu, F-75005, Paris, France

    Maria-Tereza Imaizumi & Klaus Scherrer

Authors
  1. Sohan P. Modak
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  2. Maria-Tereza Imaizumi
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  3. Monique Chappuis
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  4. Klaus Scherrer
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Modak, S.P., Imaizumi, MT., Chappuis, M. et al. Preparative density gradient centrifugation of RNA and DNA in cesium sulfateurea mixture. Mol Biol Rep 4, 55–60 (1978). https://doi.org/10.1007/BF00775182

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  • DOI: https://doi.org/10.1007/BF00775182

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