Abstract
The hydrophobic, potentially SH cross-linking reagent, phenylarsine oxide (PhAsO), was found to induce K+ and Ca2+ effluxes from mitochondria and to accelerate the respiration rate in state 4. The hydrophobic monofunctional electrophilic agent,N-ethylmaleimide, does not exhibit this effect but prevents the action of PhAsO. The polar potentially SH cross-linking reagents (arsenite, diamide) induce ion fluxes only in the presence of Pi. Ion fluxes induced by the SH reagents are inhibited by butylhydroxytoluene (an inhibitor of free radical reactions), andN,N′-dicyclohexylcarbodiimide, not by oligomycin. It is inferred that the induction of ion fluxes in mitochondria caused by cross-linking of two juxtaposed SH groups is related to the development of free radical reactions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PhAsO:
-
phenylarsine oxide
- NEM:
-
N-ethylmaleimide
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid
- RR:
-
ruthenium red
- CCCP:
-
carbonyl cyanide-m-chlorophenylhydrazone
- BHT:
-
butylhydroxytoluene
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- DTNB:
-
5,5′-dithio-bis-2-nitrobenzoic acid
- diamide:
-
diazenedicarboxylic acid-bis-dimethyl-amide
- mersalyl:
-
O-[3-hydroxymercuri)-2-methoxypropyl) carbamoylphenoxyacetic acid
- DTE:
-
dithioerythritol
References
Azzi, A., Casey, R. P., and Nalecz, M. J. (1984).Biochim. Biophys. Acta 768, 209–226.
Brierley, G. P., Settlemire, C. T., and Knight, V. A. (1967).Biochem. Biophys. Res. Commun. 28, 420–425.
Broekemeier, K. M., Schmid, P. G., Schmid, H. H. O., and Pfeiffer, D. R. (1985).J. Biol. Chem. 260, 105–113.
Diwan, J. J., De Lucia, A., and Rose, P. E. (1983).J. Bioenerg. Biomembr. 15, 277–288.
Diwan, J. J. (1982).J. Bioenerg. Biomembr. 14, 15–22.
Diwan, J. J., and Lehrer, P. H. (1978).Membr. Biochem. 1, 43–60.
Fonyo, A. and Vignais, P. V. (1980).J. Bioenerg. Biomembr. 12, 137–149.
Garlid, K. D., Di Resta, D. J., Beavis, A. D., and Martin, W. H. (1986).J. Biol. Chem. 261, 1529–1535.
Gaudemer, Y., and Latruffe, N. (1975).FEBS Lett. 54, 30–34.
Gauthier, L. M., and Diwan, J. J. (1979).Biochem. Biophys. Res. Commun. 87, 1072–1079.
Gornal, A. G., Bardawill, C. C., and David, M. M. (1949).J. Biol. Chem. 177, 751–766.
Harris, E. J., and Achenjang, F. M. (1977).Biochem. J. 168, 129–132.
Hicks, M., and Gebicki, J. M. (1981).Arch. Biochem. Biophys. 210, 56–63.
Johnson, D., and Lardy, H. (1967).Methods Enzymol. 10, 94–96.
Jung, D. W., Shi, G.-Y., and Brierley, G. P. (1980).J. Biol. Chem. 255, 408–412.
Lehninger, A. L., Vercesi, A., and Bababunmi, E. A. (1978).Proc. Natl. Acad. Sci USA 79, 1690–1694.
Martin, W. H., Beavis, A. D., and Garlid, K. D. (1984).J. Biol. Chem. 259, 2062–2065.
Novgorodov, S. A., Dragunova, S. F., and Yaguzhinsky, L. S. (1982).Biofizika (USSR) 27, 244–248.
Novgorodov, S. A., Marshansky, V. N., and Yaguzhinsky, L. S. (1984).Biochimiya (USSR) 49, 185–192.
Rasheed, B. K., Diwan, J. J., and Sanadi, D. R. (1984).Eur. J. Biochem. 144, 643–647.
Roos, I., Crompton, M., and Carafoli, E. (1980).Eur. J. Biochem. 110, 319–325.
Rugolo, M., Siliprandi, D., Siliprandi, N., and Toninello, A. (1981).Biochem. J. 200, 481–486.
Sanadi, D. R. (1982).Biochim. Biophys. Acta 683, 39–56.
Sanadi, D. R., Hughes, J. B., and Saroj, J. (1981).J. Bioenerg. Biomembr. 13, 425–431.
Siliprandi, N., Siliprandi, D., Bindoli, A., and Toninello, A. (1978). InFunctions of Glutathione in Liver and Kidney (Sies, H., and Wendel, A., eds.), Springer-Verlag, Berlin, pp. 139–147.
Siliprandi, N., Rugolo, M., Siliprandi, D., Toninello, A., and Zoccarato, F. (1979). InInternational Symposium on Function and Molecular Aspects of Biomembrane Transport (Quaglieriello, E., Palmieri, F., Papa, S., and Klingenberg, M., eds.), Elsevier-North Holland, Amsterdam, pp. 147–155.
Southard, J. H., and Green, D. E. (1974).Biochem. Biophys. Res. Commun. 61, 1310–1316.
Vignais, P. M., Chalert, J., and Vignais, P. V. (1975). InBiomembranes: Structure and Function, Vol. 34, Budapest, pp. 307–313.
Warhurst, J. W., Dawson, A. P., and Selwyn, M. J. (1982).FEBS Lett. 149, 249–252.
Wolkowicz, P. E., and McMillin Wood, J. (1981).Arch. Biochem. Biophys. 209, 408–422.
Zoccarato, F., Rugolo, M., Siliprandi, D., and Siliprandi, N. (1981).Eur. J. Biochem. 114, 195–199.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Novgorodov, S.A., Kultayeva, E.V., Yaguzhinsky, L.S. et al. Ion permeability induction by the SH cross-linking reagents in rat liver mitochondria is inhibited by the free radical scavenger, butylhydroxytoluene. J Bioenerg Biomembr 19, 191–202 (1987). https://doi.org/10.1007/BF00762412
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00762412