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Anaerobic energy metabolism in isolated adductor muscle of the sea musselMytilus edulis L.

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Summary

Energy metabolism during anaerobiosis was investigated in the isolated posterior adductor muscle of the sea mussel. Metabolism appeared to be similar to that observed in the intact organism. Glycogen and aspartate are simultaneously utilized and levels of alanine, succinate, strombine and octopine increase. The sum of the adenylates remains constant, whereas phosphoarginine is dephosphorylated. The influence of iodoacetate, aminooxyacetate and hadacidin, inhibitors of glycolysis, transamination and purine nucleotide cycle, respectively, on the utilization of substrates and the interconversion of metabolites has been studied. The results suggest that the purine nucleotide cycle is not involved in the inverse correlation of changes in levels of aspartate and alanine, but that this exclusively depends on transamination reactions. Pyruvate (required for alanine formation) arises about equally from glycolysis and aspartate decarboxylation. When the utilization of aspartate is blocked by aminooxyacetate, glycolytically formed pyruvate is metabolized by reductive condensation with glycine and arginine to yield strombine and octopine. Under this condition phosphoarginine is dephosphorylated at a faster rate in order to maintain the energy status of the cell.

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Abbreviations

Ac :

acetate

AEC :

Atkinson energy charge

Ala :

alanine

Asp :

aspartate

Glu :

glutamate

Lac :

lactate

Mal :

malate

Oct :

octopine

PA :

phosphoarginine

Prop :

propionate

Pyr :

pyruvate

Str :

strombine

Suc :

succinate

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Authors and Affiliations

  1. Laboratory of Chemical Animal Physiology, State University of Utrecht, 8 Padualaan, NL-3508 TB, Utrecht, The Netherlands

    A. de Zwaan, A. M. T. de Bont & A. Verhoeven

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  1. A. de Zwaan
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  2. A. M. T. de Bont
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  3. A. Verhoeven
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de Zwaan, A., de Bont, A.M.T. & Verhoeven, A. Anaerobic energy metabolism in isolated adductor muscle of the sea musselMytilus edulis L.. J Comp Physiol B 149, 137–143 (1982). https://doi.org/10.1007/BF00735724

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