Abstract
The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneousp-nitrophenyl-β-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 µm and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.
Similar content being viewed by others
References
Fransson LÅ (1987)Trends Biochem Sci 12:406–11.
Ruoslahti E (1988)Annu Rev Cell Biol 4:229–55.
Heinegård D, Oldberg Å (1989)FASEB J 3:2042–51.
Fransson LÅ (1989) InHeparin (Lane DA, Lindahl U, eds), pp 115–33. London: Edward Arnold.
Gallagher JT, Lyon M (1989) InHeparin (Lane DA, Lindahl U, eds), pp 135–58. London: Edward Arnold.
Lindahl U (1989) InHeparin (Lane DA, Lindahl U, eds), pp 159–89. London: Edward Arnold.
Mörgelin M, Paulsson M, Malstrom A, Heinegård D (1989)J Biol Chem 264:12080–90.
Zimmermann DR, Ruoslahti E (1989)EMBO J 8:2975–81.
Choi HU, Johnson TL, Pal S, Tang LH, Rosenberg L, Neame P (1989)J. Biol Chem 264:2876–84.
Scott JE, Orford CR (1981)Biochem J 197:213–16.
Krusius T, Ruoslahti E (1986)Proc. Natl Acad Sci USA 83:7683–87.
Fisher L, Termine JD, Young MF (1989)J Biol Chem 264:4571–76.
Göster L. Carlstedt I, Malmström A (1979)Biochem J 183:669–81.
Cöster L. Fransson LÅ (1981)Biochem J 193:143–53.
Uldbjerg N, Malmström A, Ekman G, Sheehan J, Ulmsten U, Wingerup L (1983)Biochem J 209:497–503.
Cöster L, Fransson LÅ, Sheehan J, Nieduszynski IA, Phelps CF (1981)Biochem J 197:483–90.
Tollefsen DM, Peacock ME, Monafo WJ (1986)J Biol Chem 261:8854–58.
Malmström A (1984)J Biol Chem 259:161–65.
Göster L, Nernnäs J, Malmström A (1990)Biochem J, in press.
Rice KG, Rottink MK, Linhardt RJ (1987)Biochem J 244:515–22.
Turnbull JE, Gallagher JT (1988)Biochem J 251:597–608.
Fransson LÅ, Havsmark B, Silverberg I (1990)Biochem J 269:381–88.
Lindblom A, Carlstedt I, Fransson LÅ (1989)Biochem J 261:145–53.
Schmidtchen A, Carlstedt I, Malmström A, Fransson, LÅ (1990)Biochem J 265:289–300.
Fransson LÅ, Carlstedt I (1974)Carbohydr Res 36:349–58.
Shively JE, Conrad HE (1976)Biochemistry 15:3932–42.
Laemmli UK (1970)Nature 227:680–85.
Malmström A, Carlstedt I, Åberg L, Fransson LÅ (1975)Biochem J 151:477–89.
Hjerpe A, Antonopoulos CA, Engfeldt B, Nurminen M (1982)J Chromatogr 242:193–95.
Kyhse-Andersen J (1984)J Biochem Biophys Meth 10:203.
Kolset SO, Ehlorsson J, Kjellén L, Lindahl U (1986)Biochem J 238:209–16.
Avraham S, Stevens RL, Gartner MC, Austen KF, Lalley PA, Weis JH (1988)J Biol Chem 263:7292–96.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fransson, LÅ., Schmidtchen, A., Cöster, L. et al. Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts. Glycoconjugate J 8, 108–115 (1991). https://doi.org/10.1007/BF00731020
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00731020