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Basidiomycetousras cDNA functionally replaces its homolog genes in yeast

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Abstract

It was shown by a plasmid exchange procedure that the Ras-encoding cDNA of the basidiomyceteLentinus edodes (namedLeras cDNA) can functionally replace its homolog genes (ScRAS1 andScRAS2) in the yeastSaccharomyces cerevisiae to maintain the viability of an yeast strain containing genetic disruptions of bothRAS genes. The strain replaced by aLeras−cDNA-carrying plasmid, however, grew slower than the strains replaced by aScRAS1− or aScRAS2−carrying plasmid. The intracellular level of cAMP in the strain harboring theLeras−cDNA-carrying plasmid was clearly higher than that of a parental strain which maintains a plasmid carrying theS. cerevisiae cAMP-dependent protein kinase catalytic subunit C1 gene,TPK1, but was lower than that in a strain harboring anScRAS2−carrying plasmid. These results suggest that theLeras cDNA can complement theras1 − ras2− mutation of yeast by virture of the stimulation of adenylate cyclase activity, although the complementation is not as efficient as that obtained by expressing theScRAS2 gene.

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Author notes

  1. Osamu Ishibashi

    Present address: International Research Laboratories, Ciba-Geigy (Japan) Ltd., 10-66 Miyuki-cho, 665, Takarazuka-shi, Japan

Authors and Affiliations

  1. Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midori-ku, 227, Yokohama, Japan

    Osamu Ishibashi & Kazuo Shishido

Authors
  1. Osamu Ishibashi
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  2. Kazuo Shishido
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Communicated by K. Esser

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Ishibashi, O., Shishido, K. Basidiomycetousras cDNA functionally replaces its homolog genes in yeast. Curr Genet 25, 30–33 (1994). https://doi.org/10.1007/BF00712963

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  • DOI: https://doi.org/10.1007/BF00712963

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