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Alternate energy transduction routes in chemically skinned rabbit psoas muscle fibres: a further study of the effect of MgATP over a wide concentration range

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Summary

Complex stiffness data were studied over an extended range of MgATP concentrations (3 µm-5mm) in single fibres of Ca2+-activated, chemically skinned adult rabbit psoas. The data were analysed in terms of a model involving three exponential processes, the presence of which was previously observed in fully activated muscles. As fibres were transferred from a rigor solution into solutions of gradually increasing MgATP concentration, the three processes appeared sequentially, each with a uniqueK m. The order of appearance as MgATP increases is (1) the slowest of the three processes [designated process (A)], (2) the fastest of the three processes [designated (C)], and (3) process (B), which occupies the middle range of frequencies; theK ms are approximately 10 µm, 0.2mm, and 0.8mm, respectively. The single phase advance [process (A)] remaining at very low substrate concentrations was found to be better described by a distribution of rate constants than by a single rate constant. The influence of substrate concentration on these processes is examined and two parallel hydrolysis routes are suggested as a possible mechanism.

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Authors and Affiliations

  1. Muscle Physiology Division, Department of Neurology, Columbia University, 630 West 168th Street, 10032, New York, New York, USA

    Robert N. Cox & Masataka Kawai

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  1. Robert N. Cox
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  2. Masataka Kawai
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Cox, R.N., Kawai, M. Alternate energy transduction routes in chemically skinned rabbit psoas muscle fibres: a further study of the effect of MgATP over a wide concentration range. J Muscle Res Cell Motil 2, 203–214 (1981). https://doi.org/10.1007/BF00711870

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