Abstract
A pseudomonad has been isolated from sewage, which can utilize 3-chlorobenzoic acid as a sole carbon source. In cells grown on benzoate the enzymes of the β-ketoadipic acid pathway are present. Considerable enzymic activities for chlorinated substrates were found in benzoate grown cells only for the oxygenation of 3-chlorobenzoate and the dehydrogenation of 3- and 5-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid. 3-Chlorobenzoate grown cells show additional high activities for the turnover of 3- and 4-chlorocatechols and chloromuconic acids.
Similar content being viewed by others
Abbreviations
- DHB:
-
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (derived from the trivial name, dihydrodihydroxybenzoate)
- 3- and 5-Cl-DHB correspondingly:
-
3- and 5-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid
References
Ahmed, M., Focht, D. D.: Degradation of polychlorinated biphenyls by two species ofAchromobacter. Canad. J. Microbiol.19, 47–52 (1973)
Bergmann, J. G., Sanik, J., Jr.: Determination of trace amounts of chlorine in naphtha. Analyt. Chem.29, 241–243 (1957)
Catelani, D., Colombi, A., Sorlini, C., Treccani, V.: Metabolism of biphenyl: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate: the meta-cleavage product from 2,3-dihydroxybiphenyl byPseudomonas putida. Biochem. J.134, 1063–1066 (1973)
Evans, W. C., Smith, B. S. W., Fernley, H. N., Davies, J. I.: Bacterial metabolism of 2,4-dichlorophenoxyacetate. Biochem. J.122, 543–551 (1971)
Gaunt, J. K., Evans, W. C.: Metabolism of 4-chloro-2-methylphenoxyacetate by a soil Pseudomonad. Ring-fission, lactonizing and delactonizing enzymes. Biochem. J.122, 533–542 (1971)
Holding, A. J., Collee, J. G.: Routine biochemical tests. In: Methods in microbiology, Vol. 6A, pp. 1–32, J. R. Norris, and D. W. Ribbons, Eds. London: Academic Press 1971
Horvath, R. S., Alexander, M.: Cometabolism of m-chlorobenzoate by anArthrobacter. Appl. Microbiol.20, 254–258 (1970)
Horvath, R. S.: Cometabolism of the herbicide 2,3,6-trichlorobenzoate. J. Agr. Food Chem.19, 291–293 (1971)
Horvath, R. S.: Microbial co-metabolism and the degradation of organic compounds in nature. Bact. Rev.36, 146–155 (1972)
Johnston, H. W., Briggs, G. G., Alexander, M.: Metabolism of 3-chlorobenzoic acid by a Pseudomonad. Soil Biol. Biochem.4, 187–190 (1972)
Knackmuss, H.-J., Reineke, W.: Der Einfluß von Chlorsubstituenten auf die Oxygenierung von Benzoat durchAlcaligenes eutrophus B 9. Chemosphere2, 225–230 (1973)
Ohmori, T., Ikai, T., Minoda, Y., Yamada, K.: Utilization of polyphenyls and polyphenyl-related compounds by microorganisms. Agr. Biol. Chem.37, 1599–1605 (1973)
Ornston, L. N.: The conversion of catechol and protocatechuate to β-ketoadipate byPseudomonas putida. J. biol. Chem.241, 3795–3799 (1966)
Pfennig, N., Lippert, K. D.: Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch. Mikrobiol.55, 245–256 (1966)
Reiner, A. M.: Metabolism of aromatic compounds in bacteria: Purification and properties of the catechol-forming enzyme, (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (NAD+) oxidoreductase (decarboxylating). J. biol. Chem.247, 4960–4965 (1972)
Sistrom, W. R., Stanier, R. Y.: The mechanism of formation of β-ketoadipate by bacteria. J. biol. Chem.210, 821–836 (1954)
Stanier, R. Y., Ornston, L. N.: The β-ketoadipate pathway. Advanc. Microbiol. Phys.9, 89–151 (1973)
Stanier, R. Y., Palleroni, N. J., Doudoroff, M.: The aerobic pseudomonads: A taxonomic study. J. gen. Microbiol.43, 159–271 (1966)
Wacek, A., Fiedler, R.: Über die Oxydation des Brenzcatechins zu Muconsäure. M. Chem.80, 170–185 (1949)
Willstätter, R., Müller, H. E.: Über Chlorderivate des Brenzcatechins und des o-Chinons. Chem. Ber.44, 2182–2191 (1911)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dorn, E., Hellwig, M., Reineke, W. et al. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch. Microbiol. 99, 61–70 (1974). https://doi.org/10.1007/BF00696222
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00696222