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Temperature effects on intra- and extracellular acid-base status in the American locust,Schistocerca nitens

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Summary

This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust,Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C−1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C−1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation ofP CO2 and [HCO −3 ], with changes in [HCO −3 ] predominating. Digestive tracts contained a large portion of total body water (over 30%), and total body CO2 (over 40%). Variation in [HCO −3 ] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.

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Abbreviations

C car :

carcass total CO2

C hem :

hemolymph total CO2

C in :

intracellular total CO2

C tot :

total CO2

DMO :

5,5 dimethyl-2,4-oxazolidinedione

ECW :

extracellular water mass

ECWF :

fraction of total body water which is extracellular

ICW :

intracellular water mass

ICWF :

fraction of total body water which is intracellular

PCA :

perchloric acid

S :

dissolved CO2

TBW :

total body water

TV :

tracheal volume

References

  • Bridges CR, Scheid P (1982) Buffering and CO2 dissociation of body fluids in the pupa of the silkworm moth,Hyalophora cecropia. Resp Physiol 48:183–197

    Google Scholar 

  • Brown DA, Halliwell JY (1972) Intracellular pH in rat isolated superior cervical ganglia in relation to nicotine-depolarization and nicotine-uptake. Br J Pharmacol 45:349–359

    Google Scholar 

  • Buck J, Friedman S (1958) Cyclic CO2 release in diapausing pupae-III CO2 capacity of the blood: carbonic anhydrase. J Insect Physiol 2:52–60

    Google Scholar 

  • DeJours P (1966) Respiration. Oxford University Press, New York

    Google Scholar 

  • Edwing RD, Clegg JS (1969) Lactate dehydrogenase activity and anaerobic metabolism during embryonic development inArtemia salina. Comp Biochem Physiol 31:297–307

    Google Scholar 

  • Fridericia LS (1920) Exchange of chloride ions and of carbon dioxide between blood corpuscles and blood plasma. J Biol Chem 42:245–257

    Google Scholar 

  • Gaillard S, Malan A (1985) Intracellular pH-temperature relationships in a water breather, the crayfish. Molec Physiol 7:1–16

    Google Scholar 

  • Glass ML, Boutilier RG, Heisler N (1985) Effects of body temperature on respiration, blood gases and acid-base status in the turtleChrysemys picta bellii. J Exp Biol 114:37–51

    Google Scholar 

  • Hamilton AG (1964) The occurrence of periodic or continuous discharge of carbon dioxide by male desert locusts (Schistocerca gregaria Forskal) measured by an infra-red gas analyzer. R Soc Lond Proc Series B 160:373–395

    Google Scholar 

  • Hanrahan JW, Meridith J, Phillips JE, Brandys D (1984) Methods for the study of transport and control in insect hindgut. In: Bradley TJ, Miller TA (eds) Measurement of ion transport and metabolic rate in insects. Springer, Berlin Heidelberg New York, pp 19–67

    Google Scholar 

  • Harrison JM (in press) Temperature effects on haemolymph acid-base status in vivo and in vitro in the two-striped grass-hopperMelanoplus bivittatus. J Exp Biol

  • Heisler N (1984) Acid-base regulation in fishes. In: Hoar WS and Randall DJ (eds) Fish physiology, vol XA. Academic Press, London, pp 315–401

    Google Scholar 

  • Howell BJ, Baumgarder FW, Bondi K, Rahn H (1970) Acid-base balance in cold-blooded vertebrates as a function of body temperature. Am J Physiol 218:600–606

    Google Scholar 

  • Jackson DC (1982) Strategies of blood acid-base control in ectothermic vertebrates. In: Taylor CR, Johansen K, Bolis L (eds) A companion to animal physiology. Cambridge University Press. Cambridge, pp 73–90

    Google Scholar 

  • Kurkdjian A, LeGuay JJ, Guern J (1978) Measurement of intracellular pH and aspects of its control in higher plants cultivated in liquid medium. Resp Physiol 33:75–89

    Google Scholar 

  • Layne E (1957) Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3:448–454

    Google Scholar 

  • Levenbrook L, Clark AM (1950) The physiology of carbon dioxide transport in insect blood II. The effect of insect blood on the rate of hydration of CO2. J Exp Biol 27:175–183

    Google Scholar 

  • Levy RI, Schneiderman HA (1966) Discontinuous respiration in insects II. The direct measurement and significance of changes in tracheal gas composition during the respiratory cycle of silkworm pupae. J Insect Physiol 12:83–104

    Google Scholar 

  • Lighton JRB (1985) Mihimum cost of transport and ventilatory patterns in 3 African beetles. Physiol Zool 58:390–399

    Google Scholar 

  • Loewe R, Brauer de Eggert H (1979) Blood gas analysis and acid-base status in the hemolymph of a spider (Eurypelma californicum) — Influence of temperature. J Comp Physiol 134:331–338

    Google Scholar 

  • Loughton BG, Tobe SS (1969) Blood volume in the African migratory locust. Can J Zool 47:1333–1336

    Google Scholar 

  • Miller PL (1981) Ventilation in active and inactive insects. In: Herreid CF, Fourtner CR (eds) Locomotion and energetics in arthropods. Plenum, New York, pp 367–390

    Google Scholar 

  • Miller RB, Tyson I, Relman AS (1963) pH of isolated resting skeletal muscle and its relation to potassium content. Am J Physiol 204:1048–1054

    Google Scholar 

  • Perry SF, Daxboeck C, Emmett B, Hochachka PW, Brill RW (1985) Effects of temperature change on acid-base regulation in skipjack tuna (Katsuwonus pelamis) blood. Comp Biochem Physiol 81A:49–53

    Google Scholar 

  • Phillips JE, Hanrahan J, Chamberlin M, Thomson B (1986) Mechanisms and control of reabsorption in insect hindgut. Adv Insect Physiol 19:330–422

    Google Scholar 

  • Ponten U, Siesjo BK (1964a) A method for the determination of the total carbon dioxide content of frozen tissues. Acta Physiol Scand 60:297–308

    Google Scholar 

  • Ponten U, Siesjo BK (1964b) Acid-labile carbon dioxide of rat brain after freezing the tissue in situ. Acta Physiol Scand 60, 309–317

    Google Scholar 

  • Reeves RB (1976) The interaction of body temperature and acid-base status: pH and PCO2 in a binary buffer. J Appl Physiol 40:752–761

    Google Scholar 

  • Reeves RB (1977) The interaction of body temperature and acid-base balance in ectothermic vertebrates. Annu Rev Physiol 39:559–586

    Google Scholar 

  • Roos A, Boron WF (1981) Intracellular pH. Physiol Rev 61:296–434

    Google Scholar 

  • Sokal RR, Rohlf FJ (1981) Biometry, 2nd edn. WH Freeman, San Francisco

    Google Scholar 

  • Stewart PA (1981) How to understand acid-base. A quantitative acid-base primer for biology and medicine. Elsevier, New York

    Google Scholar 

  • Storey KB, Miceli M, Butler KW, Smith ICP, Deslauriers R (1984)31P-NMR studies of the freeze-tolerant larvae of the gall fly,Eurosta solidaginis. Br J Biochem 142:591–595

    Google Scholar 

  • Thomson RB, Speight JD, Phillips JE (1988) Rectal acid secretion in the desert locust,Schistocerca gregaria. J Insect Physiol 34:829–837

    Google Scholar 

  • Truchot JP (1978) Mechanism of extracellular acid-base regulation as temperature changes in decapod crustaceans. Respir Physiol 33:161–176

    Google Scholar 

  • Waddell WJ, Butler TC (1959) Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO). Application to skeletal muscle of the dog. J Clin Invest 38:720–729

    Google Scholar 

  • Weast RC (1977) Handbook of chemistry and physics, 57th edn. CRC Press, Cleveland

    Google Scholar 

  • Weis-Fogh T (1964) Functional design of the tracheal system of flying insects as compared with the avian lung. J Exp Biol 41:207–227

    Google Scholar 

  • Wood CM, Cameron JN (1985) Temperature and the physiology of intracellular and extracellular acid-base regulation in the blue crabCallinectes sapidus. J Exp Biol 114:151–179

    Google Scholar 

Download references

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Author notes

  1. Jon M. Harrison

    Present address: Department of Zoology, University of British Columbia, V6T 2A9, Vancouver, B.C., Canada

Authors and Affiliations

  1. Department of Environmental, Population, and Organismic Biology, University of Colorado, 80309-0334, Boulder, Colorado, USA

    Jon M. Harrison

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  1. Jon M. Harrison
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Harrison, J.M. Temperature effects on intra- and extracellular acid-base status in the American locust,Schistocerca nitens . J Comp Physiol B 158, 763–770 (1989). https://doi.org/10.1007/BF00693015

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  • DOI: https://doi.org/10.1007/BF00693015

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