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Immunohistological evidence of perivascular localization of basic protein in early development of experimental allergic encephalomyelitis

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Summary

The early histological changes in the CNS, functional state of the blood-brain barrier, production of humoral anti-brain immunoglobulins and immunohistological changes in myelin sheaths have been studied in two groups of guinea pigs inoculated with encephalitogenic antigens (basic protein, homologous and heterologous brain) mixed with two different doses of adjuvant. All antigens mixed with a low dose of adjuvant induced histological changes in about 20% of the animals. The blood-brain barrier was not or only slight disturbed. Antigens mixed with a high dose of adjuvant provoked severe EAE in 95% of the animals. The blood-brain-barrier displayed multiple injuries with diffusion of anti-brain antibodies into the nervous tissue. Antibody production showed no significant dependence on the different antigens and the various doses of adjuvant. The immunohistological examination using antibasic protein serum revealed the abolition of fluorescence within areas of inflammatory infiltrates and/or within areas of diffusion of antibodies. The perivascular accumulation of basic protein occurs early preceding the formation of perivascular infiltrates. It is proposed that this probably endogenous basic protein represents the first target structure, which can easily be trapped by circulating sensitized lymphocytes and/or antibodies. In this way it acts as a trigger of early lesions in EAE.

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Authors and Affiliations

  1. Laboratory of Immunopathology, Department of Neuromorphology, Max Planck Institute for Psychiatry, Munich

    Jiri Simon & Archinto P. Anzil

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  1. Jiri Simon
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  2. Archinto P. Anzil
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Simon, J., Anzil, A.P. Immunohistological evidence of perivascular localization of basic protein in early development of experimental allergic encephalomyelitis. Acta Neuropathol 27, 33–42 (1974). https://doi.org/10.1007/BF00687238

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  • DOI: https://doi.org/10.1007/BF00687238

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