Summary
Intravenously (i.v.) injected horseradish peroxidase (HRP) which has leaked out of the vessels in a cryogenic cortical injury of adult mice is taken up into a large number of neurons resulting in two different forms of labeling.
Diffuse neuronal labeling of, the type previously reported in many conditions with vasogenic brain edema occurred particularly within the primary lesion. The other and more frequent type, here calledgranular neuronal labeling, was present in a wide zone immediately outside the injury. Such neurons contained HRP in numerous cytoplasmic granules and had the same characteristics as normal neurons accumulating HRP after retrograde axonal transport. By using highly sensitive histochemical methods for demonstration of HRP we could also follow bundles of labeled axons out from the primary lesion. Some of them passed the corpus callosum to the fronto-parietal cortex of the contralateral hemisphere.
With this report we would like to put emphasize on certain phenomena occurring in neurons which previously have not been particularly recognized in studies on vasogenic brain edema. It can be assumed that in a focal brain lesion components from the edematous fluid and other “wound substances” can be taken up into nerve cell processes and then be intracellularly transported in different directions. In this way, nerve cell populations located in other brain areas and even in the contralateral hemisphere may be influenced by components from the primary injury.
References
Becker NH, Hirano A, Zimmerman HM (1968) Observations of the distribution of exogenous peroxidase in the rat cerebrum. J Neuropathol Exp Neurol 27:439–452
Eccles JC (1981) The modular operation of the cerebral neocortex considered as the material basis of mental events. Neuroscience 6:1839–1856
Flodmark I (1965) Blood-brain barrier alteration after experimental cold injury of the rabbit brain, indicated by penicillin G in EEG and by dye tests. Acta Physiol Scand 63:225–235
Hass GM, Taylor BC (1948) A quantitative hypothemal method for the production of local injury of tissue. Arch Pathol 45:563–580
Klatzo I (1979) Cerebral edema and ischemia. Rec Adv Neuropathol 1:27–41
Klatzo I, Piraux A, Laskowski EJ (1958) The relationship between edema, blood-brain barrier and tissue elements in a local brain injury. J Neuropathol Exp Neurol 17:548–564
Kristensson K (1978) Retrograde transport of macromolecules in axons. Ann Rev Pharmacol Toxicol 18:97–110
Kristensson K, Olsson Y (1971) Retrograde axonal transport of protein. Brain Res 29:363–365
LaVail JH, LaVail MM (1972) Retrograde axonal transport in the central nervous system. Science 176:1416–1417
Malmgren LT, Olsson Y (1978) A sensitive method for light microscopical demonstration of horseradish peroxidase Brain Res 148:279–294
Mesulam M-M (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117
Olsson Y, Hossmann K-A (1970) Fine structural localization of exudated protein tracers in the brain. Acta Neuropathol (Berl) 16:103–116
Rosene PL, Mesulam M-M (1978) Fixation variables in horseradish peroxidase neurohistochemistry. I. The effects of fixation time and perfusion procedures upon enzyme activity. J Histochem Cytochem 26:28–39
Szentágothai J (1978) The neuron network of the cerebral cortex: A functional interpretation. Proc R Soc Lond B 201:219–248
Author information
Authors and Affiliations
Additional information
Supported by grants from the Swedish Medical Research Council, project 12X-03020 and Trygg-Hansa, Stokholm
Rights and permissions
About this article
Cite this article
Tengvar, C., Olsson, Y. Uptake of macromolecules into neurons from a focal vasogenic cerebral edema and subsequent axonal spread to other brain regions. Acta Neuropathol 57, 233–235 (1982). https://doi.org/10.1007/BF00685395
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00685395