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Untersuchungen von Renin und Reninsubstrat bei Schwangeren

Renin and renin substrate in pregnancy

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Summary

Simultaneous determinations of renin and renin substrate (angiotensinogen) were carried out in 25 normotensive pregnant women during late stages of gestation. For comparison a group of 10 normotensive healthy non pregnant women of comparable age were investigated.—All determinations of renin and renin substrate were done at the same time. To each sample renin or renin substrate, respectively, was added in excess. The procedure was then followed through in a modified manner according to the micro-method of Boucher et al. for determination of renin; purification of renin substrate was done on plasma from nephrectomized sheep.

The results show that circulating angiotensinogen is increased more than threefold late in pregnancy. Furthermore the ratio of renin activity/renin substrate (angiotensinogen) is significantly elevated during pregnancy.—The results are reviewed in the light of current concepts pertinent to the etiology of high blood pressure in toxemia of pregnancy.

Zusammenfassung

Bei 25 normotonen Schwangeren wurden in der Spätschwangerschaft Parallelbestimmungen von Renin und Angiotensinogen (Reninsubstrat) im Plasma durchgeführt. Zum Vergleich diente eine Gruppe von 10 normotonen, gesunden, nichtschwangeren Frauen gleichen Alters. Die Bestimmung von Renin und Reninsubstrat wurden im gleichen Ansatz unter Zusatz des jeweils beteiligten anderen Wirkstoffs im Überschuß durchgeführt. Die Aufarbeitung erfolgte mit einer Modifikation der von Boucher et al. angegebenen Mikromethode unter Verwendung von angereichertem Substrat aus dem Plasma nephrektomierter Schafe.

Die Untersuchungen zeigen, daß das zirkulierende Angiotensinogen in der Spätschwangerschaft um mehr als das Dreifache erhöht ist. Das Verhältnis von Reninaktivität zu Angiotensinogen (Reninsubstrat) in der Schwangerschaft ebenfalls signifikant erhöht. Die Befunde werden in Verbindung mit den Theorien über die Entstehung des Bluthochdrucks bei Schwangerschaftstoxikosen kritisch diskutiert.

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Authors and Affiliations

  1. Universitäts-Frauenklinik Mainz, Mainz

    A. -W. Schmidt & J. Rosenthal

  2. Dept. of Hypertension, University Hospital, Boston, Mass., USA

    A. -W. Schmidt & J. Rosenthal

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  1. A. -W. Schmidt
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  2. J. Rosenthal
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Mit Unterstützung der Deutschen Forschungsgemeinschaft.

Fräulein Helgard Messer und Frau A. Vescei danken wir für die zuverlässige und kritische technische Mitarbeit.

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Schmidt, A.W., Rosenthal, J. Untersuchungen von Renin und Reninsubstrat bei Schwangeren. Arch. Gynak. 212, 20–29 (1972). https://doi.org/10.1007/BF00668005

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