Summary
The effects of stimulation of α and β adrenergic receptors on short circuit current (S.C.C.), Na+ and Cl− fluxes and osmotic water permeability were studied on isolated frog skin epithelial layers separated from the dermis.
Low norepinephrine doses (final concentrations in the incubation medium ranging from 5×10−9 to 10−8 M) produced increased water permeability and S.C.C. The latter was entirely accounted for by an increase in the active Na+ influx. Na+ outflux and Cl− fluxes were not modified. Both these effects disappeared after treatment with the β blocking agent, Propranolol. Higher norepinephrine doses (final concentrations: 10−7 to 10−6 M) produced: 1. an increase in water permeability lower than that produced by low doses, the highest doses failing to increase water permeability, and 2. a triphasic change in S.C.C.: after an initial increase, S.C.C. dropped to its resting value and then rose again to a sustained value. Na+ and Cl− flux measurements showed that the variation in S.C.C. reflected variations in active Na+ transport. When the same high norepinephrine doses were applied after treatment with the α blocking agent Phentolamine, the effects observed were identical to those obtained with low doses.
On β blocked preparations, large doses of norepinephrine inhibited the water permeability and sodium transport increases induced by theophylline or oxytocin but did not modify those induced by 3′5′-cyclic AMP. The inhibition was suppressed after blocking α receptors.
From the foregoing, it was concluded that both α and β adrenergic receptors are present in frog skin epithelial cells and are involved in the regulation of water and sodium permeability.
It is suggested that the inhibitory effect of α stimulation resulted from the inhibition of cyclic-AMP generating system, the activity of which is under the positive control effect of oxytocin and β stimulation.
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Rajerison, R.M., Montegut, M., Jard, S. et al. The isolated frog skin epithelium: Presence of α and β adrenergic receptors regulating active sodium transport and water permeability. Pflugers Arch. 332, 313–331 (1972). https://doi.org/10.1007/BF00588578
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DOI: https://doi.org/10.1007/BF00588578