Summary
The left atrium of the guinea-pig, isolated functionally by ligatures from the other cardiac cavities, was perfused continuously (Krebs-Henseleit solution, O2 saturated, 37°C, pH 7.4) through the pulmonary veins and stimulated artificially (at a rate of 120/min) during a period of 8 h. Isovolumetric pressure amplitudes were recorded each 1/2 h at various rates of stimulation (60 to 240/min).
Results. Frequency potentiation is not pronounced within the first 1/2 h after isolation, but develops almost to its full extent after 5 h of perfusion, mainly owing to a decline of the amplitudes at lower rates, combined with a prolongation in peak time and with a decay of the rates of pressure change during the rising and falling phase of contraction. Noradrenaline added after 71/2 h to the perfusion fluid (10−6 mol/l), restores almost completely the contractile state of the preparation, as observed in the first records after isolation. A possible correlation between the depletion of noradrenaline stores and the development of frequency potentiation is discussed.
Similar content being viewed by others
References
Abbott, B. C., Mommaerts, W. F. H. M.: A study of inotropic mechanisms in the papillary muscle preparation. J. gen. Physiol.42, 533–551 (1959)
Buddenbrock, W. v.: Vergleichende Physiologie, Bd. VI, S. 189. Basel-Stuttgart: Birkhäuser 1967
Dhalla, N. S., Bhagat, B. D., Sulakhe, P. V., Olson, R. E.: Catecholamine stores of the isolated rat heart perfused with substrate-free medium. J. Pharmacol. exp. Ther.177, 96–101 (1971)
Gilmore, J. P., Nizolek, J. A., Jacob, R. J.: Further characterization of myocardial K+-loss induced by changing contraction frequency. Amer. J. Physiol.221, 465–469 (1971)
Graham, T. P., Powell, W. J., Jr., Clancy, R. L., Gilmore, J. P.: Characterization of the frequency-force relationship in the dog atrium in situ. Circulat. Res.20, 338–348 (1967)
Kahn, M., Kavaler, F., Fisher, V. J.: Frequency-force relationship of canine ventricular muscle in vivo and in vitro. In preparation (1974)
Kavaler, F., Harris, R. S., Lee, R. J., Fisher, V. J.: Frequency-force behaviour of in-situ ventricular myocardium in the dog. Circulat. Res.28, 533–544 (1971)
Kilz, U., Schaefer, J., Van Zwieten, A.: The contribution of adrenergic mechanisms to frequency potentiation and to paired stimulation in guinea-pig isolated atrial tissue. Pflügers Arch.308, 203–213 (1969)
Koch-Weser, J.: Role of norepinephrine release in the interval-strength relationship of the heart muscle. J. Pharmacol. exp. Ther.150, 184–189 (1965)
Koch-Weser, J., Blinks, J. R.: The influence of the interval between beats on muocardial contractility. Pharmacol. Rev.15, 601–652 (1963)
Langer, G. A.: Ion fluxes in cardiac excitation and contraction and their relation to myocardial contractility. Physiol. Rev.48, 708–757 (1968)
Langer, G. A., Brady, A. J.: Potassium in dog ventricular muscle: Kinetic studies of distribution and effects of varying frequency of contraction and potassium concentration of perfusate. Circulat. Res.18, 164–177 (1966)
Levy, J. V., Richards, V.: The influence of reserpine pretreatment on the contractile and metabolic effects produced by ouabain on isolated rabbit left atria. J. Pharmacol. exp. Ther.147, 205–211 (1965)
Lindmar, R., Muscholl, E.: Die Wirkung von Cocain, Guanethidin, Reserpin, Hexamethonium, Tetracain und Psicain auf die Noradrenalin-Freisetzung aus dem Herzen. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.240, 214–227 (1961)
Reichel, H.: Kritik der Muskelmodelle und ihrer Anwendbarkeit auf das Myokard des Warmblüters. Intern. Symp. “Das chronisch kranke Herz”, Bad Krozingen 18.–22. 10. 1972
Reichel, H., Rumberger, E., Schaefer, J., Schwarzkopf, H. J., Baumann, K.: Frequency-potentiation in the human myocardium. Basic Res. Cardiol.69, 11–20 (1974)
Reiter, M.: Differences in the inotropic cardiac effects of noradrenaline and dihydroouabain. Naunyn-Schmiedeberg's Arch. Pharmacol.275, 243–250 (1972)
Reiter, M., Seibel, K., Stickel, F. J.: Sodium dependence of the inotropic effect of a reduction in extracellular potassium concentration. Naunyn-Schmiedebergs Arch. Pharmak.268, 361–378 (1971)
Rosin, H., Farah, A.: Post-stimulation potentiation of contractility in the isolated auricle of the rabbit. Amer. J. Physiol.180, 75–82 (1955)
Spilker, B., Cervoni, P.: Electrical membrane activity and frequency-force relations in normal, denervated and reserpine-pretreated cat heart muscle. J. Pharmacol. exp. Ther.168, 60–69 (1969)
Trautwein, W., Dudel, J.: Aktionspotential und Mechanogramm des Warmblüterherzmuskels als Funktion der Schlagfrequenz. Pflügers Arch. ges. Physiol.260, 24–39 (1954)
Trautwein, W., Kassebaum, D. G., Nelson, R. M., Hecht, H. H.: Electrophysiological study of human heart muscle. Circulat. Res.10, 306–312 (1962)
Tritthart, H., Fleckenstein, A., Kaufmann, R.: Die spezifische Beschleunigung des Erschlaffungsprozesses durch sympathische Überträgerstoffe und die Hemmung dieses Effektes durch β-Receptoren-Blockade. Pflügers Arch.303, 350–365 (1968)
Tuttle, R. S., Farah, A.: The effect of ouabain on the frequency-force relation and on post-stimulation potentiation in isolated atrial and ventricular muscle. J. Pharmacol. exp. Ther.135, 142–150 (1962)
Williams, J. F., Sonnenblick, E. H., Braunwald, E.: Determinants of atrial contractile force in the intact heart. Amer. J. Physiol.209, 1061–1068 (1965)
Author information
Authors and Affiliations
Additional information
This study has been supported by the “Stiftung Volkswagenwerk” and the “Deutsche Forschungsgemeinschaft”.
Rights and permissions
About this article
Cite this article
Baumann, K., Reichel, H. Time dependence of frequency potentiation in the isolated guinea-pig's atrium. Pflugers Arch. 350, 69–80 (1974). https://doi.org/10.1007/BF00586739
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00586739