Abstract
The contraction kinetics of smooth muscle show a down-regulation after the transient rise found during sustained contraction. We tried to find out therefore if the contraction kinetics of rat tracheal smooth muscle can be re-accelerated during sustained activation. A 2 s length vibration (100 Hz sinusoidal; amplitude=6% of the muscle length) produces an immediate fall in the force developed by the activated muscle. A biexponential function was fitted to the force recovery. The reciprocal of the time constant,t 2, describing the slow component of force recovery, reflects the kinetics of contraction. The contraction kinetics reach their highest levels (t 2=4.9±0.1 s,n=166) about 30 s after the onset of electrical field stimulation. Three experimental groups were activated by either 10 μM serotonin (5-HT), 100 μM acetylcholine (ACh), or by 2 μM ACh for 50 min. Approximately 10 vibrations were applied to each preparation after an 8 min activation in order to observe stabilized down-regulated contraction kinetics.t 2 values were calculated from the force recovery after vibration and averaged 11.2±0.2 s (n=141), 11.5±0.2 s (n=137), and 11.1±0.3 s (n=84), respectively. After 50 min of continuous chemical activation, the preparation was stimulated additionally by the neurogenic release of acetylcholine. Thet 2 of post-vibration force recovery, as measured after 30 s of neural activation, showed no change in the specimens basically activated by 100 μM ACh (11.0±0.4 s,n=51). A decline int 2, indicating accelerated kinetics, was observed in the groups which had been stimulated by 10 μM 5-HT (5.9±0.2 s,n=51) and 2 μM ACh (5.6±0.2 s,n=47). The re-accelerating effect of the second stimulus could be reproduced recurrently. The down-regulated contraction kinetics can be re-accelerated either by activating another receptor type in addition to the one already maximally stimulated or by increasing the stimulus mediated by one of the receptor types from half maximal to maximal strength. However, this is only possible if the additional activation is strong enough, as indicated by an increase in active force. It could be demonstrated that the slowing of the cross-bridge cycling rate is the result of a regulatory process and not the result of substrate deficiencies or refractoriness in the regulatory of contractile proteins.
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Aksoy MO, Murphy RA, Kamm KE (1982) Role of Ca2+ and myosin light chain phosphorylation in regulation of smooth muscle. Am J Physiol 242:C109-C116
Aksoy MO, Mras S, Kamm KE, Murphy RA (1983) Ca2+, cAMP, and changes in myosin phosphorylation during contraction of smooth muscle. Am J Physiol 245:C255-C270
Arner A (1982) Mechanical characteristics of chemically skinned guinea pig taenia coli. Pflügers Arch 395:277–284
Arner A, Hellstrand P (1983) Activation of contraction and ATPase activity in intact and chemically skinned smooth muscle of rat portal vein. Circ Res 53:695–702
Arner A, Hellstrand P (1985) Effect of calcium and substrate on force-velocity relation and energy turnover in skinned smooth muscle of the giinea-pig. J Physiol (Lond) 369:347–365
Brenner B (1980) Effect of free sarcoplasmatic Ca2+-concentration on maximum unloaded shortening velocity: measurements on single glycerinated rabbit psoas fibres. J Muscle Res Cell Motil 1:409–428
Chatterjee M, Murphy RA (1983) Calcium-dependent stress maintenance without myosin phosphorylation in skinned smooth muscle. Science 221:464–466
De Lanerolle P, Condit JR, Tanenbaum M, Adelstein RS (1982) Myosin phosphorylation, agonist concentration and contraction of tracheal smooth muscle. Nature 298:871–872
Dillon PF, Murphy RA (1982) Tonic force maintenance with reduced shortening velocity in arterial smooth muscle. Am J Physiol 242:C102-C108
Dillon PF, Aksoy MO, Driska SP, Murphy RA (1981) Myosin phosphorylation and the cross-bridge cycle in arterial smooth muscle. Science 211:495–497
Fay FS, Shlevin HH, Granger WC, Taylor SR (1979) Aequorin luminescence during activation of single isolated smooth muscle cells. Nature 280:506–508
Gagelmann M, Güth K (1985) Force generated by non-cycling cross-bridges at low ionic strength in skinned smooth muscle from taenia coli. Pflügers Arch 403:210–214
Galvas PE, Kuettner C, Paul RJ, DiSalvo J (1985) Temporal relationship between isometric force, phosphorylase, and protein kinase activities in vascular smooth muscle. Proc Soc Exp Biol Med 178:254–260
Gerthoffer WT (1986) Calcium dependence of myosin phosphorylation and airway smooth muscle contraction and relaxation. Am J Physiol 250:597–604
Gerthoffer WT, Murphy RA (1983a) Myosin phosphorylation and regulation of cross-bridge cycle in tracheal smooth muscle. Am J Physiol 244:C182-C187
Gerthoffer WT, Murphy RA (1983b) Ca2+, myosin phosphorylation, and relaxation of arterial smooth muscle. Am J Physiol 245:C271-C277
Haeberle JR, Hott JW, Hathaway DR (1985) Regulation of isometric force and isotonic shortening velocity by phosphorylation of the 20,000 dalton myosin light chain of rat uterine smooth muscle. Pflügers Arch 403:215–219
Hill AV (1938) The heat of shortening and the dynamic constants of muscle. Proc R Soc B 126:136–195
Kamm KE, Stull JT (1985) Myosin phosphorylation, force and maximal shortening velocity in neurally stimulated tracheal smooth muscle. Am J Physiol 249:C238-C247
Klemt P, Peiper U, Speden RN, Zilker F (1981) The kinetics of post-vibration tension recovery of the isolated rat portal vein. J Physiol (Lond) 312:218–296
Krisanda JM, Paul RJ (1984) Energetics of isometric contraction in porcine carotid artery. Am J Physiol 246:C510-C519
Morgan JP, Morgan KG (1983) Vascular smooth muscle: The first recorded Ca2+ transients. Pflügers Arch 395:75–77
Morgan JP, Morgan KG (1983) Differential effects of vasoconstrictors on intracellular Ca2+-levels in mammalian vascular smooth muscle as detected with aequorin. Fed Proc 42:571
Paul RJ, Doerman G, Zeugner C, Rüegg JC (1983) The dependence of unloaded shortening velocity on Ca2+, calmodulin, and duration of contraction in chemically skinned smooth muscle. Circ Res 53:342–351
Peiper U, Vahl CF, Donker E (1984) The time course of changes in contraction kinetics during the tonic activation of the rat tracheal smooth muscle. Pflügers Arch 402:83–87
Peiper U (1983) Alterations in smooth muscle contraction kinetics during tonic activation. Pflügers Arch 399:203–207
Persechini A, Stull JT, Cooke R (1984) The effect of myosin phosphorylation on the contractile properties of skinned rabbit muscle fibers. J Biol Chem 260:7951–7954
Rembold CM, Murphy RA (1986) Myoplasmic calcium myosin phosphorylation, and regulation of the crossbridge cycle in swine arterial smooth muscle. Circ Res 58:803–815
Siegman MJ, Butler TM, Mooers SU, Davies RE (1980) Chemical energetics of force development, force maintenance and relaxation in mammalian smooth muscle. J Gen Physiol 76:609–629
Siegman MJ, Butler TM, Mooers SU, Michalek A (1984) Ca2+ can effectV max without changes in myosin light chain phosphorylation in smooth muscle. Pflügers Arch 401:385–390
Siegman MJ, Butler TM, Mooers SU (1985) Energetics and regulation of cross-bridge states in mammalian smooth muscle. Experientia 41:1020–1025
Siegman MJ, Butler TM, Mooers SU (1986) The use of prestimulation and variable relaxation times to probe the regulation of crossbridge states in mammalian smooth muscle. J Muscle Res Cell Motil 7:379–380
Silver PJ, Stull JT (1982) Regulation of myosin light chain phosphorylation in tracheal smooth muscle. J Biol Chem 257:6145–6150
Silver PJ, Stull JT (1983) Phosphorylation of myosin light chain and phosphorylase in tracheal smooth muscle in response to KCl and carbachol. Mol Pharmacol 25:267–274
Stephenson RP (1956) A modification of receptor theory. Br J Pharmacol 11:379–393
Sumimoto K, Kuriyama H (1986) Mobilisation of free Ca2+ measured during contraction-relaxation cycles in smooth muscle cells of the porcine coronary artery using quin 2. Pflügers Arch 406:173–180
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Lobnig-Meier, B.M., Peiper, U. & Zimmermann, A. Re-acceleration of the down-regulated contraction kinetics in the rat tracheal smooth muscle. Pflugers Arch. 410, 413–419 (1987). https://doi.org/10.1007/BF00586519
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DOI: https://doi.org/10.1007/BF00586519