Abstract
Intracellular microelectrode techniques were used together with inhibitors of Na+ transport (amiloride) and H+ transport (acetazolamide and SITS1) to identify principal cells and intercalated cells in the outer stripe of the rabbit outer medullary collecting duct. The principal cell (n=9) had a basolateral membrane voltage (V bl) of −64.7±3.2 mV, a fractional resistance of the apical membrane (fR a=R a/R a+R bl) of 0.82±0.02, and a K+-selective basolateral membrane. Luminal amiloride hyperpolarizedV bl by 10.3±2.1 mV and increasedfR a to near unity (n=7). Bath acetazolamide and SITS were without effect on these parameters. The intercalated cell (n=5) had aV bl of −25.0±3.2 mV, afR a of 0.99±0.01, and a Cl−-selective basolateral membrane. Bath acetazolamide or SITS hyperpolarizedV bl by 26.4±8.2 mV. Luminal amiloride did not alterV bl of this cell. The differential effects of the inhibitors also indicate that the principal and intercalated cells are probably not directly coupled electrically.
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Koeppen, B.M. Electrophysiological identification of principal and intercalated cells in the rabbit outer medullary collecting duct. Pflugers Arch. 409, 138–141 (1987). https://doi.org/10.1007/BF00584761
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DOI: https://doi.org/10.1007/BF00584761