Abstract
Plasma Na+, erythrocyte Na+ content and the activity of Na+ transport systems of red cells were measured in Wistar rat fed a normal or high Na+ diet. Net Na+ and K+ fluxes of erythrocytes were also measured in the presence of plasma of rats fed with excess Na+.
Na+−K+ cotransport and passive Na+ permeability were increased. Erythrocyte Na+ content was increased after 2 months but not after 8 days of high Na+ intake. No significant difference in plasma Na+ and pump activity could be detected after such a diet. No factor acting in Na+ extrusion was found to be present in plasma of salt loaded rats. These results indicate that Na+ intake may modulate Na+ transport systems, namely passive permeability and Na+−K+ cotransport and that increased Na+ erythrocyte content is not a causative factor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aperia A, Larsson L, Zetterström R (1981) Hormonal induction of Na, K ATPase in developing proximal tubular cells. Am J Physiol 241:F356–F360
Aragon I, Montenay-Garestier T, Devynck MA, Pernollet MG, Nunez AM, Ben-Ishay D, Meyer P, Helene C (1982) Diffuse structural abnormalities in cell membranes from genetically hypertensive rats: a fluorescence polarization study. Clin Sci 63:49s-52s
Beaugé LA, Ortiz O (1971) Sodium and rubidium fluxes in rat red blood cells. J Physiol 218:533–549
Beilin LJ, Knight GJ, Munro-Faure AD, Anderson S (1966) The sodium potassium and water contents of red blood cells of healthy human adults. J Clin Invest 45:1817–1825
Canessa M, Adragna N, Solomon HS, Connolly TM, Tosteson DC (1980) Increased sodium-lithium countertransport in red cells of patients with essential hypertension. New Engl J Med 302:772–776
Carrasco L, Smith AE (1976) Sodium ions and the shut-off of ghost cell protein synthesis by picornaviruses. Nature 264:807–809
Dagher G, Garay RP (1980) The maximal rate of outward Na+,K+ cotransport in erythrocytes from essential hypertensive patients. Can J Biochem 58:1069–1074
Dahl LK (1977) Salt intake and hypertension. In: Genest J, Koiw E, Kuchel O (eds) Hypertension. Mc Graw Hill, New York, pp 548–559
Davidson J, Opie L, Keding B (1982) Sodium potassium cotransport activity as genetic marker in essential hypertension. Br Med J 284:539–541
De Luise M, Blackburn GL, Flier JS (1980) Reduced activity of the red cell sodium potassium pump in human obesity. New Engl J Med 303:1017–1022
De Mendonça M, Grichois ML, Toumi K, Herubel A, Knorr A, Garay R, Meyer P (1981) Furosemide and bumetanide sensitive Na+ fluxes in erythrocyte from genetically hypertensive rats (SHR). Clin Exp Hyper 3:885–895
De Mendonça, M, Garay RP, Ben-Ishay D, Meyer P (1981) Abnormal erythrocyte cation transport in primary hypertension. Hypertension 3:I179–I183
De Mendonça M, Knorr A, Grichois ML, Ben-Ishay D, Garay RP, Meyer P (1982) Erythrocytic sodium ion transport systems in primary and secondary hypertension of the rat. Kidney Intern 21:S69–S75
De Mendonça M, Grichois ML, Toumi K, Knorr A, Guicheney P, Dagher G, Garay RP, Meyer P, Ben-Ishay D (1983) Erythrocyte Na+ handling in rat genetic hypertension. In: Transport across membrane and hypertension. Villareal H. J. Wiley, New York [in press]
De Pirro R, Fusco A, Lauro R, Testa I, Ferreti F, De Martinis C (1980) Erythrocyte insulin receptors in non insulin dependent diabetes mellitus. Diabetes 29:96–99
Devynck MA, Pernollet MG, Matthews PG, Mac Donald GJ, Raisman RS, Meyer P (1979) Sodium intake and plasma angiotensin level as modulators of adrenal and uterine angiotensin receptors in the rat. J Cardiovasc Pharmacol 1:163–179
Devynck MA, Pernollet MG, Nunez AM, Aragon I, Montenay-Garestier T, Helene C, Meyer P (1982) Diffuse structural alterations in cell membranes of spontaneously hypertensive rats. Proc Natl Acad Sci 79:5057–5060
De Wardener HE (1982) The natriuretic hormone. Quarterly J Exp Physiol 67:371–376
Doucet A, Katz AI (1980) Renal potassium adaptation: Na−K ATPase activity along the nephron after chronic potassium loading. Am J Physiol 238:F380–F386
Duhm J (1982) Lithium transport pathways in erythrocytes. In: De Lux HD, Aldenhoff MD, Ehrich H (eds) Basic mechanisms in the action of lithium. Excerpta Medica, pp 21–27
Duhm J, Göbel BO (1982) Sodium-lithium exchange and sodium-potassium cotransport in human erythrocytes. Part 1: Evaluation of a simple uptake test to assess the activity of the two transport systems. Hypertension 4:468–476
Duhm J, Göbel BO, Beck FX (1982) Na+−K+ cotransport and volume of erythrocytes of hypertensive and K+ deficient rats. Pflügers Arch 394:R28
Ellory JC, Flatman PW, Stewart GW (1980) Inhibition of human red cell sodium and potassium influxes by external divalent cations. J Physiol 307:37–38
Garay RP, Adragna N, Canessa M, Tosteson D (1981) Outward Na+,K+ cotransport in human red cells. J Membr Biol 62:169–174
Glynn I, Karlish (1975) The sodium pump. Ann Rev Physiol 37:13–55
Haddy FJ, Pamnani MB, Clough DL (1980) Role of humoral factor in low renin hypertension. In: Lichardus B, Schrier RW, Ponec J (eds) Hormonal regulation of sodium excretion. Elsevier, North Holland, pp 379–385
Hoffman JE (1966) The red cell membrane and the transport of sodium and potassium. Am J Med 41:666–679
Kaplay SS (1979) Modified kinetics of erythrocyte membrane Na+,K+ adenosine triphosphatase in protein energy malnutrition. Bioch Med 22:282–287
Kornel L (1981) Studies on the mechanism of mineralocorticoid induced hypertension: evidence for the presence of an in situ mechanism in the arterial wall for a direct action of mineralocorticoids. Clin Bioch 14:282–293
McPartland RP, Rapp JP (1982) (Na+−K+) activated adenosine triphosphatase and hypertension in Dahl salt sensitive and resistant rats. Clin Exp Hypertension 3:379–391
Meneely GR, Tucker RG, Darby WS, Auerbach SH (1974) Chronic sodium chloride toxicity in the albino rat. Occurrence of hypertension and a syndrome of oedema and renal failure. J Exp Med 98:71–79
Morgan T, Myers J, Fitzgibbon W (1981) Sodium intake, blood pressure and red cell sodium efflux. Clin Exp Hyper 3:641–655
Murray RH, Luft FC, Block R, Weyman AE (1978) Blood pressure responses to extremes of sodium intake in normal man. Proc Soc Exp Biol Med 159:432–436
Ostrow D, Pandey G, Davis J, Hurt S, Tosteson P (1978) A heritable disorder of Li transport in erythrocyte of a subpopulation of manic depressive patients. Am J Psych 135:1070–1078
Palfrey HC, Feit P, Greengard P (1980) cAMP stimulated cation cotransport in avian erythrocytes. Proc Natl Acad Sci USA 77:1711–1715
Pamnani MB, Clough DL, Huot SJ, Haddy FS (1980) Vascular Na+−K+ pump activity in Dahl S and R rats. Proc Soc Exp Biol Med 165:440–444
Postnov YV, Orlov SN, Shevchenko A, Adler AM (1977) Altered sodium permeability, calcium binding and Na−K ATPase activity in the red blood cell membrane is essentiall hypertension. Pflügers Arch 371:263–269
Sanchez A, Pettinger WA (1981) Dietary sodium regulation of blood pressure and renal α1 and α2 receptors in WKY and SH rats. Life Sci 29:2795–2802
Wald H, Guy R, Gutman Y, Czaczkes W (1977) Sodium ammonium and unilateral nephrectomy: differential effect on microsomal ATPase of kidney cortex and medulla. Int J Bioch 8:33–37
Wiley JS, Cooper R (1974) A furosemide sensitive cotransport of sodium plus potassium in the human red cell. J Clin Invest 53:745–755
Wiley JS, Hutchinson JS, Mendelsohn FAO, Doyle AE (1980) Increased sodium permeability of erythrocytes in spontaneously hypertensive rats. Clin Exp Pharmacol Physiol 7:527–530
York DA, Bray GA, Yukinura Y (1978) An enzymatic defect in the obese (ob/ob) mouse: loss of thyroid included sodium and potassium dependent adenosine triphosphatase. Proc Natl Acad Sci 75:477–481
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
De Mendonça, M., Grichois, M.L., Dagher, G. et al. Modulation of Na+ transport systems in Wistar rat erythrocytes by excess dietary Na+ intake. Pflügers Arch. 398, 64–68 (1983). https://doi.org/10.1007/BF00584715
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00584715