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Evaluation of vasogenic edema in experimental brain tumors by cathodoluminescence and fluorescence microscopy

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Summary

Cathodoluminescence and fluorescence microscopy have been used to study vasogenic edema in experimentally induced brain tumors in rats. Both methods are suited for the demonstration of FITC- or TRITC-coupled antiserum, and thus allow the evaluation of serum protein extravasation. Cathodoluminescence is more time consuming and laborious than fluorescence microscopy, but it has distinct advantages: Contrast enhancement improves the differentiation between certain cell types, and the higher resolution of the scanning electron microscope allows the identification of subcellular regions which cannot be recognized by conventional fluorescence microscopy.

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Authors and Affiliations

  1. Forschungsstelle für Hirnkreislaufforschung, Max-Planck-Institut für Hirnforschung, Ostmerheimer Straße 200, D-5000, Köln 91, Federal Republik of Germany

    T. Hürter, W. Bröcker & K.-A. Hossmann

  2. Institut für Medizinische Physik, Universität Münster, Hüfferstraße 68, D-4400, Münster, Federal Republik of Germany

    T. Hürter, W. Bröcker & K.-A. Hossmann

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  1. T. Hürter
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  2. W. Bröcker
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  3. K.-A. Hossmann
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Hürter, T., Bröcker, W. & Hossmann, KA. Evaluation of vasogenic edema in experimental brain tumors by cathodoluminescence and fluorescence microscopy. Histochemistry 72, 249–254 (1981). https://doi.org/10.1007/BF00517138

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  • DOI: https://doi.org/10.1007/BF00517138

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