Summary
Bombesin, a tetradecapeptide isolated from the skin of some European discoglossid frogs, has been reported previously to reduce renal blood flow and glomerular filtration rate and to increase plasma renin activity in anaesthetized dogs.
In the present study bombesin was infused intravenously in anaesthetized dogs at dose levels of 3, 6 and 12 ng/kg/min for 6 h and renal blood flow, glomerular filtration rate, oxygen consumption, oxygen extraction by the kidney tissue, as well as plasma erythropoietin levels (ESF) and plasma renin activity were measured. Plasma levels of ESF increased during bombesin infusion only when renal blood flow was reduced to a level of 1 ml/g/min or less. In this situation glomerular filtration was blocked, renal oxygen consumption was decreased to 10% of normal and oxygen extraction by the kidney was increased by 2 times. No correlation was found between plasma renin activity and ESF concentrations during bombesin infusion. It is concluded that the stimulant action of bombesin on ESF production is a consequence of the renal hypoxia induced by the reduction in renal blood flow.
Similar content being viewed by others
References
Anastasi, A., Erspamer, V., Bucci, M.: Isolation and amino acid sequences of alytesin and bombesin, two analogues active tetradecapeptides from the skin of European discoglossid frogs. Arch. Biochem. Biophys. 148, 443–446 (1972)
Deetjen, P., Kramer, K.: Die Abhängigkeit des O2-Verbrauchs der Niere von der Na-Rückresorption. Pflügers Arch. ges. Physiol. 273, 636–650 (1961)
DeGowin, R. L., Hofstra, D., Gurney, C. W.: The mouse with hypoxia-induced erythremia, an erythropoietin bioassay animal. J. Lab. clin. Med. 60, 846–852 (1962)
Erspamer, V., Melchiorri, P., Sopranzi, N.: The action of bombesin on the kidney of the anaesthetized dog. Brit. J. Pharmacol. 48, 438–455 (1973)
Fisher, J. W., Samuels, A., Langston, J. W.: Effects of angiotensin and renal artery constriction on erythropoietin production. J. Pharmacol. exp. Ther. 157, 618–625 (1967)
Fisher, J. W., Taylor, G., Porteous, D. D.: Localization of erythropoietin in glomeruli in sheep kidney by fluorescent antibody technique. Nature (Lond.) 205, 611–612 (1962)
Fried, W., Gurney, C. W.: Use of mild plethora to demonstrate an erythropoietic effect from small amounts of androgens. Proc. Soc. exp. Biol. (N.Y.) 120, 519–521 (1965)
Long, J. S., Severs, W. B.: Renin release from the ischaemic kidney. Brit. J. Pharmacol. 48, 97–105 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Melchiorri, P., Sopranzi, N. & Roseghini, M. Effects of bombesin on erythropoietin production in the anaesthetized dog. Naunyn-Schmiedeberg's Arch. Pharmacol. 294, 193–197 (1976). https://doi.org/10.1007/BF00507853
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00507853