Summary
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1.
The effect of short tetanic stimulation (30 Hz for 25 s) on the following twitch responses of the myenteric plexus-longitudinal muscle preparation of guinea-pig ileum to electric stimulation (0.1 Hz) was investigated in the presence of naloxone and indomethacin.
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2.
Post-tetanic potentiation (PTP) of the twitches observed in control experiments was abolished in preparations desensitized by substance P but it was not affected in preparations desensitized by serotonin or pretreated with methysergide.
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3.
Immediately after 5 min tetanic stimulation a decreased sensitivity to substance P but unchanged sensitivity to serotonin were observed.
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4.
Electromyogram (EMG) of the longitudinal muscle layer was picked up 4 and 10 mm aborally from the stimulation site in response to 1 to 16 impulse trains delivered at 100 Hz. In control conditions only the longer trains triggered this neurogenic response at the distal recording site. In the presence of substance P but not serotonin facilitation occured so that the distal site was frequently recruited to respond with an EMG even to single impulses.
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5.
A substance P-like compound rather than serotonin may be a candidate for the neuromodulator or neurotransmitter substance involved in PTP and changes in the response topography of muscarinic transmission.
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Kadlec, O., Mašek, K. & Šeferna, I. Post-tetanic potentiation at the nerve-muscle junction in the longitudinal muscle of the guinea-pig ileum. Naunyn-Schmiedeberg's Arch. Pharmacol. 326, 262–267 (1984). https://doi.org/10.1007/BF00505328
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DOI: https://doi.org/10.1007/BF00505328