Summary
Glucose and cellobiose are the principle sugars released during cellulose hydrolysis. The utilization of mixtures of these sugars (10 g·l−1) by Candiada wickerhamii in batch cultures under aerobic and non-aerated conditions was investigated. Glucose was utilized first followed by cellobiose after a diauxic lag. Ethanol was produced from glucose under both aerobic and non-aerated conditions. Following glucose depletion under aerobic conditions, ethanol (produced during growth on glucose) and cellobiose were utilized simultaneously. Under non-aerated conditions ethanol was produced from cellobiose after the diauxic lag. When the glucose concentration was increased from 2 to 8 g·l−1 in glucose: cellobiose mixtures (total sugar 10 g·l−1), decreases of 66–91% were observed in the specific rates of growth, ethanol production, β-glucosidase production and sugar utilization on cellobiose as well as an increase in the diauxic time lag under aerobic and non-aerated conditions. After depletion of glucose, the viability of C. wickerhamii decreased during cellobiose utilization.
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Abbreviations
- E:
-
β-glucosidase activity (U · ml−1)
- q maxp :
-
Maximum specific rate of ethanol production [g ethanol · (g dry biomass · h)−1] or [g ethanol · (cell · h)−1]
- q maxs :
-
Maximum specific rate of substrate utilization [g substrate · (g dry biomass · h)−1]
- q maxe :
-
Maximum specific rate of β-glucosidase production [U · (g dry biomass · h)−1]
- U:
-
Aryl-β-glucosidase activity (μmol p-nitrophenol released · min−1)
- X:
-
Dry biomass (g · l−1)
- μ:
-
specific growth rate (h−1)
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Kilian, S.G., Prior, B.A. & Lategan, P.M. Diauxic utilization of glucose-cellobiose mixtures by Candida wickerhamii . European J. Appl. Microbiol. Biotechnol. 18, 369–373 (1983). https://doi.org/10.1007/BF00504747
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DOI: https://doi.org/10.1007/BF00504747