Summary
A new medium (MBS) for optimal sporulation of Bacillus sphaericus was defined. With the two main mosquito pathogenic strains grown in this medium, 1593-4 and 2297, highest cell and spore yields were obtained, concomitantly with an highest larvicidal activity against Culex pipiens. Study of both strains asporulated mutants showed a decrease in larvicidal power. After plasmid curing treatments, toxicity of strain 1593-4 did not decrease, neither toxic parasporal inclusion bodies of strain 2297 disappear.
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Kalfon, A., Larget-Thiéry, I., Charles, JF. et al. Growth, sporulation and larvicidal activity of Bacillus sphaericus . European J. Appl. Microbiol. Biotechnol. 18, 168–173 (1983). https://doi.org/10.1007/BF00498040
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DOI: https://doi.org/10.1007/BF00498040