Summary
Renal biopsy has been performed on three young and healthy human subjects. A light microscopic investigation of material fixed in formalin, embedded in paraffin and stained with Ladewig's modification of the Mallory staining shows in each of two of the patients severe changes of one glomerulus. These changes are probably inflammatory reactions and are similar to what is observed in subacute glomerulonephritis. Changes are also observed in the glomeruli of the third patient but they are so slight that they could not be regarded as definite lesions. The authors presume that these changes in the glomeruli are due to an allergic inflammation evoked by inflammations of the throat.
Elektron microscopic investigation of other parts of the same biopsy material shows considerable modifications of the usual structure of the capillary epithelial cells. The mitochondria are enlarged and in the cytoplasm a number of very large vesicles appear. It is presumed that these changes are reversible and that they are connected with a change of function of the glomeruli with an increased uptake of fluid in the cells. It cannot be excluded, however, that these changes are irreversible and are signs of an incipient destruction of cells during the filtration through the capillary wall.
The authors emphasize that it is very important that changes of this kind may occur in healthy subjects and that one may not draw any definite conclusions from their presence in biopsy material from patients with different diseases; at least, if only single glomeruli are damaged.
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References
Bergstrand, A.: Electron microscopic investigations of the renal glomeruli. Lab. Invest. 6, 191–204 (1957).
Bergstrand, A., and H. Bucht: Electron microscopic investigation on biopsy material from patients with renal diseases. A case of subacute glomerulonephritis. Electron Microscopy. Proc. of the Stockholm Conference, Sept. 1956, p. 256–258.
—: Electron microscopic investigations on the glomerular lesions in Diabetes mellitus (diabetic glomerulosclerosis). Lab. Invest. 6, 293–300 (1957).
Farquhar, M. G., R. L. Vernier and R. A. Good: Studies on familial nephrosis II. Amer. J. Path. 33, 791–805 (1957a).
—, The application of electron microscopy in pathology: Study of renal biopsies. Schweiz. med. Wschr. 87, 501–510 (1957b).
Gansler, H., et C. Rouiller: Modifications physiologiques et pathologiques du chondriome. Schweiz. Z. allg. Path. 19, 217–243 (1956).
Kark, R. M., and R. C. Muerhcke: Biopsy of kidney in prone position. Lancet 1954 I, 1047–1049.
McGregor, L.: Histology of normal glomerulus. Amer. J. Path. 5, 545–558 (1929).
Miller, F., and A. Bohle: Electron microscopy of the glomerular basement membrane in experimental amyloidosis of the mouse. Electron Microscopy. Proc. of the Stockholm Conference, Sept. 1956, p. 254–256.
Mueller, C. B., C. D. Mason and D. G. Stout: Anatomy of the glomerulus. Amer. J. Med. 18, 267–276 (1955).
Rhodin, J.: Correlation of ultrastructural organization and function in normal and experimentally changed proximal convoluted tubule cells of the mouse kidney. Thesis, Stockholm, 1954.
—: Electron microscopy of the glomerular capillary wall. Exp. Cell Res. 8, 572–574 (1955).
Schulz, H., H. Löw, L. Ernster u. F. S. Sjöstrand: Elektronenmikroskopische Studien an Leberschnitten von Thyroxinbehandelten Ratten. Electron Microscopy. Proc. of the Stockholm Conference, Sept. 1956, p. 134–137.
Sjöstrand, F. S.: Electron microscopy of cells and tissues. In Oster and Pollister, Physical techniques in biological research, III, p. 241–298. New York: Academic Press Inc. 1956.
— An improved method to prepare formvar nets for mounting thin sections for electron microscopy. Electron Microscopy. Proc. of the Stockholm Conference, Sept. 1956, p. 120–121.
Sjöstrand, F. S., and V. Hanzon: Unpublished data, cited by Zetterqvist.
Sjöstrand, F. S., and J. Rhodin: The ultrastructure of the proximal convoluted tubules of the mouse kidney as revealed by high resolution electron microscopy. Exp. Cell Res. 4, 426–430 (1953).
Yamada, E.: The fine structure of the glomerulus of the mouse. J. biophys. biochem. Cytol. 1, 551–565 (1955).
Zetterqvist, H.: The ultrastructural organization of the columnar absorbing cells of the mouse jejunum. Thesis, Stockholm, 1956.
Zimmermann, K. W.: Über den Bau des Glomerulus der menschlichen Niere. Z. mikr.-anat. Forsch. 18, 520–537 (1929).
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This work was aided by a grant from “Stiftelsen Therèse och Johan Anderssons Minne”.
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Bergstrand, A., Bucht, H. Anatomy of the glomerulus as observed in biopsy material from young and healthy human subjects. Z.Zellforsch 48, 51–73 (1958). https://doi.org/10.1007/BF00496712
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DOI: https://doi.org/10.1007/BF00496712