Summary
It has been shown that an isolated KOH PAS-effect of epithelial mucosubstances in the colonic mucosae of the rat can be demonstrated also without the conventionally used preliminary oxidation/reduction step. The method is based on the use of strongly diluted periodic acid after previous treatment of tissue sections with ethanolic KOH. As the positive reacting material has been proven to be sensitive against treatment with neuraminidase the modified KOH PAS-reaction should be related to the presence of acylated sialic acid residues in mucosubstances of the colonic mucosae of the rat.
Zusammenfassung
Es wird über Untersuchungen berichtet, die zeigen, daß auch ohne Oxidation/Reduktion-Technik ein isolierter KOH PAS-Effekt an epithelialen Mukosubstanzen des Rattendickdarms nachweisbar ist. Die Methode beruht auf der Verwendung stark verdünnter Perjodsäure nach vorausgehender Schnittbehandlung mit äthanolischer KOH. Da sich das mit dieser modifizierten KOH PAS-Reaktion nachweisbare Material als Neuraminidasesensitiv erwies, dürfte die Reaktion auf acylierte Sialinsäurereste in bestimmten Mukosubstanzen des Rattencolon zu beziehen sein.
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Klessen, C. Demonstration eines Alkali PAS-Effektes bei Verwendung von Perjodsäure in niedriger Konzentration. Histochemistry 56, 299–305 (1978). https://doi.org/10.1007/BF00495991
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DOI: https://doi.org/10.1007/BF00495991