Summary
Transformation of the spermatozoon of Ascaris suum from a spheroidal to an ameboid cell is associated with the formation of a motile pseudopodium and coalescence of the intracellular refringent granules. The pseudopodia of the ameboid spermatozoa contain filaments organized into dense patches, bundles, web-like or lace-like networks, as observed by electron microscopy.
The morphology and chemistry of the refringent granules were investigated in subcellular fractions enriched for these structures. Isolated refringent granules were heterogeneous in size measuring from 0.5×0.6 to 2.3×3.5 μm. Each granule is surrounded by a 110 Å thick layer. During fusion, the surfaces of the refringent granules form small extensions resembling micropodia. The process of fusion occurs at many sites on a given granule and simultanenous fusion of several granules was commonly observed.
Amino acid analyses of the refringent granule proteins (RGP's) indicated: they are rich in aspartic acid or asparagine (48%), leucine (10%), serine (19%) and aromatic amino acids (11%). Gas-liquid chromatographic analyses of alditol acetate derivatives of monosaccharides released by mild acid hydrolysis showed the predominant sugars to be glucose (7.3 μg/mg protein), galactose (9.2 μg/mg) and N-acetylglucosamine (5.5 μg/mg). Lipid analyses indicated a complex mixture of glycerides, ascarosides and waxes, together with a major component that resembled free fatty acid in mobility on TLC.
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References
Abbas MK, Cain GD (1979) In vitro activation and behavior of the ameboid sperm of Ascaris suum (Nematoda). Cell Tissue Res 200:273–294
Abbas MK, Cain GD (1980) lodination of surface components of the spheroidal and ameboid spermatozoa of Ascaris suum (Nematoda). Biol Reprod 22:1007–1014
Abbas MK, Cain GD (1981a) Surface receptors: Are they involved in transformation of spermatozoa of Ascaris? Cell Tissue Res 214:553–567
Abbas MK, Cain CD (1981b) Subcellular fractions and the refringent granules of the spermatozoa of Ascaris suum (Nematoda). Cell Tissue Res 221:125–136
Barret J (1981) Biochemistry of parasitic helminths. University Park Press, Baltimore
Clark WH, Jr, Moretti RL, Thomson WW (1972) Histochemical and ultracytochemical studies of the spermatids and sperm of Ascaris lumbricoides var. suum. Biol Reprod 7:145–159
Folch J, Lees M, Sloane-Stanely GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Foor WE (1968) Zygote formation in Ascaris lumbricoides (Nematoda). J Cell Biol 39:119–134
Hydén H (1974) A calcium-dependent mechanism for synapse and nerve cell membrane modulation. Proc Natl Acad Sci USA 71:2965–2968
Kaulenas MA, Fairbairn D (1968) RNA metabolism of fertilized Ascaris lumbricoides eggs during uterine development. Exp Cell Res 52:233–251
Liu TY, Chang YH (1971) Hydrolysis of proteins with p-toluene-sulfonic acid. J Biol Chem 246:2842–2848
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739
Moore S, Stein WH (1954) Procedures for the chromatographic etermination of animo acids on four percent cross-linked sulfonated polystyrene resins. J Biol Chem 211:893–906
Niedermeier W (1971) Gas chromatography of neutral and amino sugars in glycoproteins. Anal Biochem 40:465–475
Niedermeier W, Tomana M (1974) Gas chromatographic analysis of hexosamines in glycoproteins. Anal Biochem 57:363–368
Panijel J (1950) Recherches sur la nature et la signification e la protéine gram da gamete mâl d'Ascaris megalocephala. Biochim Biophys Acta 6:79–93
Tarr GE, Fairbairn D (1973a) Glycerides, waxes and sterols in ovaries of Ascaris lombricoides (Nematoda). Lipids 8:303–310
Tarr GE, Fairbairn D (1973b) Conversion of ascaroside esters to free ascarosides in fertilized eggs of Ascaris suum. J Parasitol 59:428–433
Van Beneden E, Julin C (1984) La spermatogénèse chez l'ascaride megalocephale. Bull Acad Roy Belg 7:312–342
Williams MA, McCluer RH (1980) The use of Sep-Pak C18 cartridges during the isolation of gangliosides. J Neurochem 35:266–269
Wolf N, Hirsch D, McIntosh JR (1978) Spermatogenesis in males of the free-living nematode, Caenorhabditis elegans. J Ultrastruct Res 63:155–169
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Abbas, M.K., Cain, G.D. Amino acid and lipid composition of refringent granules from the ameboid sperm of Ascaris suum (Nematoda). Histochemistry 81, 59–65 (1984). https://doi.org/10.1007/BF00495402
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DOI: https://doi.org/10.1007/BF00495402