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Histochemical, autoradiographic and electron microscopic investigations of the renal proximal tubule of male and female rats after castration

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Summary

After castration of 90-day-old male and female rats, changes appear in the renal proximal tubule. A distinction can be made between early changes (up to 10th postoperative day) and later changes (20th–30th postoperative day). Between the 3rd and 5th day after castration the kidney of the females shows an increase in free estrogen receptors (biochemical studies) which are localized in the pars contorta of the proximal tubule (autoradiographic studies), while the male kidney shows a marked increase in urinary protein excretion up to the 10th day after castration. Proximal tubule changes detectable histochemically and electron microscopically do not appear until day 20 or 30 after castration. The results of castration are similar in segments S1 and S2. By days 20 and 30 after castration there is a decrease in the activity of lysosomal enzymes (acid phosphatase, acid β-galactosidase). Electron microscopy shows a conspicuous decrease in the number of giant lysosomes (mainly in females) and apical vacuoles (mainly in males). A marked increase in the number of lysosomes is found in the S3 segment; females always have more lysosomes than males. The number of peroxisomes is also greatly increased; they appear circular in the females but can assume bizarre shapes in the males. Lipid droplets appear in the basal region of the tubule cell of segments S2 and S3 in the males. The sex differences are preserved in all segments even after castration and become even more pronounced in the S3 segment.

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References

  • Albano SB, Rogonese P (1959) Il comportamento della fosfatasi alcalina nel fegato e nel rene del ratto in alcune condizioni fisiologiche (ciclo estrale) e sperimentali (castrazione e somministrazione di ormoni ovarici). Attual Ost Gin (Padova) 5:1045–1054

    Google Scholar 

  • Ballantyne B, Bright JE (1978) Variations in mammalian renal cortical β-glucuronidase activity according to species, strain and sex. Cell Mol Biol 23:369–377

    Google Scholar 

  • Bargmann W (1978) Geschlechtsbedingte Unterschiede der Nieren. In: Handbuch der mikroskopischen Anatomie des Menschen. Band 7: Harn-und Geschlechtsapparat. Teil 5. Springer-Verlag, Berlin Heidelberg New York, pp 331–341

    Google Scholar 

  • Barka T, Anderson PJ (1962) Histochemical methods for acid phosphatase using hexazonium pararosaniline as coupler. J Histochem Cytochem 10:231–232

    Google Scholar 

  • Barrett JM, Heidger PM (1975) Microbodies of the rat renal proximal tubule: Ultrastructural and cytochemical investigations. Cell Tissue Res 157:283–305

    Google Scholar 

  • Baulieu EE (1978a) New approaches in pharmacology and therapeutics. In: Straub RW, Bolis L (eds) Cell membrane receptors for drugs and hormones: A multidisciplinary approach. Raven Press, New York, pp 129–149

    Google Scholar 

  • Baulieu EE (1978b) Wirkungsmechanismus der Östrogene. Physiologische und pharmakologische Aspekte der hormonellen “Rezeptivität”. Die Anti-Östrogene. Klin Wochenschr 56:683–695

    Google Scholar 

  • Bourdeau JE, Carone FA (1974) Protein handling by the renal tubule. Nephron 13:22–34

    Google Scholar 

  • Bourdeau JE, Carone FA, Ganote CE (1972) Serum albumin uptake in isolated perfused renal tubules. J Cell Biol 54:382–398

    Google Scholar 

  • Burton J, Pearse A (1952) A critical study of the methods for histochemical localisation of β-glucuronidase. Br J Exp Pathol 30:87–97

    Google Scholar 

  • Carone FA, Peterson DR, Oparil S, Pulman TN (1979) Renal tubular transport and catabolism of proteins and peptides. Kidney Int 16:271–278

    Google Scholar 

  • Christy NP, Shaver JC (1974) Estrogens and the kidney. Kidney Int 6:366–376

    Google Scholar 

  • Clark JH, Peck EJ (1977) Steroid hormone receptors: Basic principles and measurement. In: O'Malley BW, Birnbaumer L (eds) Receptors and hormone action, Vol. I. Academic Press, New York, pp 383–410

    Google Scholar 

  • Daigeler R (1980) Veränderungen im Nierenhauptstück der Ratte nach Hypophysektomie. Elektronenmikroskopische Untersuchungen. Anat Anz (im Druck)

  • Davidoff M, Caffier H, Schiebler TH (1980) Steroid Hormone Binding Receptors in the Rat Kidney. Histochemistry 69:39–48

    Google Scholar 

  • Deimling O v (1970) Enzymarchitektur der Niere und Sexualhormone. Prog Histochem Cytochem 1:1–50

    Google Scholar 

  • Deimling O v, Bausch I (1967) Histochemische und quantitative Untersuchungen zur Verteilung der sauren Phosphatase in der Rattenniere. Histochemie 10:193–200

    Google Scholar 

  • Deimling O v, Noltenius H (1964) Hormonabhängige Enzymverteilung in Geweben. I. Histochemische Untersuchungen über die Geschlechtsunterschiede der alkalischen Nierenphosphatase bei erwachsenen Ratten. Histochemie 3:500–508

    Google Scholar 

  • Deimling O v, Riedel U, Klinge R, Brämswig J (1967) Hormonabhängige Enzymverteilung in Geweben. VIII. Die Phosphomonoesterasen der Rattenniere nach Exstirpation verschiedener endokriner Drüsen. Histochemie 11:234–252

    Google Scholar 

  • DeVries JR, Ludens JH, Fanestil DD (1972) Estradiol renal receptor molecules and estradioldependent antinatriuresis. Kidney Int 2:95–100

    Google Scholar 

  • E.O.R.T.C., Brest Cancer Cooperative Group EOR (1973) Standarts for assessment of estrogen receptors in human breast cancer. Eur J Cancer 9:379

    Google Scholar 

  • Ericsson JLE, Trump BF (1964) Electron microscopic studies of the epithelium of the proximal tubule of the rat kidney. Lab Invest 13:1427–1456

    Google Scholar 

  • Ericsson JLE, Trump BF, Weibel J (1965) Electron microscopic studies of the proximal tubule of the rat kidney. II. Cytosegresomes and Cytosomes: their relationship to each other and to the lysosome concept. Lab Invest 14:1341–1365

    Google Scholar 

  • Galaske RE, Van Liew JB, Feld LG (1979) Filtration and reabsorption of endogenous low-molecular-weight protein in the rat kidney. Kidney Int 16:394–403

    Google Scholar 

  • Ghraf R, Lax ER, Wagner W, Schriefers H (1977) Endocrine regulation of sex dependent hydroxysteroid dehydrogenase activities in rat kidney. J. Endocrinol 73:289–330

    Google Scholar 

  • Gomori R (1952) Microscopic histochemistry, University of Chicago Press, Chicago

    Google Scholar 

  • Gossrau R (1978) Azoindoxylverfahren zum Hydrolasennachweis. IV. Zur Eignung verschiedener Diazoniumsalze. Histochemistry 57:323–342

    Google Scholar 

  • Jacobsen NO, Jørgensen F (1973) Ultrastructural observations on the pars descendens of the proximal tubule in the kidney of the male rat. Z Zellforsch 136:479–499

    Google Scholar 

  • Katz AI, Lindheimer MD (1977) Actions of hormones on the kidney. Annu Rev Physiol 39:97–134

    Google Scholar 

  • Li JJ, Talley DJ, Li AA, Villee CA (1974) An estrogen binding protein in the renal cytosol of intact, castrated and estrogenized golden hamster. Endocrinology 95:1134–1141

    Google Scholar 

  • Lojda Z (1970) Indigogenic methods for glycosidases. An improved method for β-d-galactosidase and its application to localization studies in the intestine and in other tissue. Histochemie 23:266–288

    Google Scholar 

  • Lojda Z, Gossrau R, Schiebler TH (1979) Enzyme histochemistry. Springer-Verlag, Berlin Heidelberg New York

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Maunsbach AB (1966) Absorption of 125J-labeled homologus albumin by rat kidney proximal tubule cells. J Ultrastruct Res 15:197–241

    Google Scholar 

  • Maunsbach AB (1973) Ultrastructure of the proximal tubule. In: Orloff J, Berliner RW, Washington DC (eds) Handbook of physiology. Vol. VIII: Renal physiology. American Physiological Society, Bethesda, Maryland, pp. 31–79

    Google Scholar 

  • Maunsbach AB (1976) Cellular mechanisms of tubular protein transport. In: Thurau K (ed) Kidney and urinary tract physiology II, vol 11. University Park Press, Baltimore, pp 145–167

    Google Scholar 

  • Pantić V, Li J, Villee C (1974) Autoradiographic studies of the kidneys of hamsters bearing longterm oestrogen implants. Cytobiologie 2:89–99

    Google Scholar 

  • Rutenburg AM, Kim H, Fischbein JW, Hanker JS, Wasserkrug HL, Seligman AM (1969) γ-Glutamyl transpeptidase activity. J Histochem Cytochem 17:517–526

    Google Scholar 

  • Ryan KJ, Meigs RA, Petro Z, Morrison G (1963) Estrogen-induced 16-hydroxysteroid dehydrogenase activity in rat kidney. Science 142:243–244

    Google Scholar 

  • Scatchard G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672

    Google Scholar 

  • Schiebler TH, Danner KG (1978) The effect of sex hormones on the proximal tubules in the rat kidney. Cell Tissue Res 192:527–549

    Google Scholar 

  • Schiebler TH, Mühlenfeld E (1966) Über die geschlechtsspezifische Chemodifferenzierung der Rattenniere. Naturwissenschaften 53:311

    Google Scholar 

  • Schiebler TH, Mühlenfeld WE (1967) Über geschlechtsspezifische Unterschiede im Fermentmuster der Ratte. Anat Anz Erg.-Heft 120:41–48

    Google Scholar 

  • Sergeev PV, Dunaev VG, Nichoga VD (1978) An investigation into the effects produced by testosterone and estradiol on the activity of lysosomal enzymes of the rat liver and kidneys. Farmakol toksikol (Moskau) 41:445–450

    Google Scholar 

  • Sierociński W, Schiebler TH (1980) Geschlechtsunterschiede in der Niere verschiedener Säuger. 74. Verh Anat Ges, Anat Anz, Erg-Bd (im Druck)

  • Smirnov AN, Smirnova TA, Rozen VB (1975) The effect of extrogens on the concentration of estradiol receptors in rat uterus, kidneys and liver (Russ.). Probl Endokrinol 21:73–78

    Google Scholar 

  • Straus W (1964) Cytochemical observations on the relationship between lysosomes and phagosomes in kidney and liver by combined staining for acid phosphatase and intravenously injected horseradish peroxidase. J Cell Biol 20:497–507

    Google Scholar 

  • Stumpf W, Sar M (1975) Autoradiographic techniques for localizing steroid hormones. In: O'Malley BW, Hardman JG (eds) Methods in enzymology, Vol. XXXVI: Hormone action. Part A: Steroid hormones. Academic Press, New York, pp 135–157

    Google Scholar 

  • Stumpf WE, Sar M (1976) Autoradiographic localization of estrogen, androgen, progestin, and glucocorticosteroid in “target tissues” and “nontarget tissues”. In: Pasqualini I (ed) Receptors and mechanism of action of steroid hormones, Part I. Marcel Dekker, New York, pp 41–84

    Google Scholar 

  • Weissmann G (1969) The effects of steroids and drugs on lysosomes. In: Dingle JT, Fell HB (eds) Lysosomes in biology and pathology, vol. I. North-Holland, Amsterdam-London, pp 276–295

    Google Scholar 

  • Wenk H (1966) Hormonabhängige Enzymverteilung in der Niere. I. Stimulation der β-hydroxy-Buttersäure-Dehydrogenase-Aktivität bei normalen und kastrierten männlichen Albinoratten durch Oestradiol. Z mikrosk Anat Forsch 75:198–209

    Google Scholar 

  • Wenk H (1968) Hormonabhängige Enzymverteilung in der Niere. IV. Wirkung von Kastration und Sexualhormonen auf die Aktivität der β-Hydroxy-Buttersäure-Dehydrogenase in der Niere bei weiblichen Albinoratten. Histochemie 12:120–129

    Google Scholar 

  • Winckler J (1970a) Zum Einfrieren von Gewebe in Stickstoff gekühltem Propan. Histochemie 23:44–50

    Google Scholar 

  • Winckler J (1970b) Verwendung gefriergetrockneter Kryostatschnitte für histologische und histochemische Untersuchungen. Histochemie 24:168–186

    Google Scholar 

  • Zeller J (1973) Zur Cytochemie der Lysosomen in der Rattenniere unter normalen und experimentellen Bedingungen. Histochemie 35:235–262

    Google Scholar 

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Author notes

  1. M. Zabel (Research fellow of the Alexander von Humboldt Foundation)

    Present address: Department of Histology and Embryology, Academy of Medicine, Institute of Biostructure, ul. Swiecickiego 6, Poznan, Poland

Authors and Affiliations

  1. Department of Anatomy, University of Würzburg, Koellikerstraße 6, D-8700, Würzburg, Federal Republic of Germany

    M. Zabel (Research fellow of the Alexander von Humboldt Foundation) & T. H. Schiebler

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  1. M. Zabel
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  2. T. H. Schiebler
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Dedicated to Prof. Dr. W. Graumann, Department of Anatomy, University of Tübingen, on the occasion of his 65th birthday

Supported by the Deutsche Forschungsgemeinschaft (SFB 105)

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Zabel, M., Schiebler, T.H. Histochemical, autoradiographic and electron microscopic investigations of the renal proximal tubule of male and female rats after castration. Histochemistry 69, 255–276 (1980). https://doi.org/10.1007/BF00489772

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