Abstract
Electrophoresis of human tears on slab polyacrylamide gels showed five phenotypes among anodal tear proteins. These phenotypes are the expression of autosomal codominant alleles. Gene frequencies are as follows: for Caucasians, At 1=0.99, At 3=0.01; for Negroes, At 1=0.97, At 2=0.03; for Chinese, At 1=0.98, and At 4 and At 5 are both approximately 0.008.
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References
Allansmith, M. (1973). Immunology of the tears. In Holly, F. J., and Lemp, M. A. (eds.), The Preocular Tear Film and Dry Eye Syndrome, Vol. 13, No. 1 of International Ophthalmology Clinics, Little, Brown, Boston, p. 47.
Azen, E. A. (1972). Genetic polymorphism of basic proteins from parotid saliva. Science 176673.
Azen, E. A., and Oppenheim, F. G. (1973). Genetic polymorphism of proline-rich human salivary proteins. Science 1801067.
Balakrishnan, C. R., and Ashton, G. C. (1974). Polymorphism of human salivary proteins. Am. J. Hum. Genet. 26145.
Bonavida, B., Sapse, A. T., and Sercarz, E. E. (1969). Specific tear prealbumin: A unique lacrimal protein absent from serum and other secretions. Nature 221375.
Broekhuyse, R. M. (1974). Tear lactoferrin: A bacteriostatic and complexing protein. Invest. Ophthalmol. 13550.
Carmody, P. J., Rattazzi, M. C., and Davidson, R. G. (1973). Tay-Sach's disease—the use of tears for the detection of heterozygotes. New Engl. J. Med. 2891072.
Chandler, J. W., Leder, R., Kaufman, H. E., and Caldwell, J. R. (1974). Quantitative determinations of complement components and immunoglobulins in tears and aqueous humor. Invest. Ophthalmol. 13151.
Del Monte, M. A., Johnson, D. L., Cotlier, E., Krivitt, W., and Desnick, R. J. (1974). Diagnosis of Fabry's disease by tear α-galactosidase A. New Engl. J. Med. 29057.
Iwata, S. (1973). Chemical composition of the aqueous phase. In Holly, F. J., and Lemp, M. A. (eds.), The Preocular Tear Film and Dry Eye Syndrome, Vol. 13, No. 1 of International Ophthalmology Clinics, Little, Brown, Boston, p. 29.
Josephson, A. S., and Lockwood, D. W. (1964). Immunoelectrophoretic studies of the protein components of normal tears. J. Immunol. 93532.
Josephson, A. S., and Wald, A. (1969). Enhancement of lysozyme activity by anodal tear protein. Proc. Soc. Exp. Biol. Med. 131677.
Josephson, A. S., and Weiner, R. S. (1968). Studies of the proteins of lacrimal secretions. J. Immunol. 1001080.
Kueppers, F. (1971). Proteinase inhibitor in human tears. Biochim. Biophys. Acta 229845.
Poulik, M. D. (1959). Starch-gel immunoelectrophoresis. J. Immunol. 82502.
Rennert, O. M., Kaiser, D., Sollberger, H., and Joller-Jemelka, S. (1974). Anti-protease activity in tears and nasal secretions. Humangenetik 2373.
Sapse, A. T., Bonavida, B., Stone, W., Jr., and Sercarz, E. E. (1969). Proteins in human tears. I. Immunoelectrophoretic patterns. Arch. Ophthalmol. 81815.
Schultze, H. E., and Heremans, J. F. (1966). Molecular Biology of Human Proteins, Vol. 1, Elsevier, Amsterdam, Chap. 10.
Singer, J. D., Cotlier, E., and Krimmer, R. (1973). Hexoseaminidase A in tears and saliva for rapid identification of Tay-Sach's disease and its carriers. Lancet 21116.
Tapasztó, I. (1973). Pathophysiology of human tears. In Holly, F. J., and Lemp, M. A. (eds.), The Preocular Tear Film and Dry Eye Syndromes, Vol. 13, No. 1 of International Ophthalmology Clinics, Little, Brown, Boston, p. 119.
Van Haeringen, N. J., and Glasius, E. (1974a). Lactate dehydrogenase in tear fluid. Exp. Eye Res. 18345.
Van Haeringen, N. J., and Glasius, E. (1974b). Enzymes of energy-producing metabolism in human tear fluid. Exp. Eye Res. 18407.
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This study was supported by a grant from the National Institutes of Dental Research (5-R01-DE-E-03658-10).
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Azen, E.A. Genetic polymorphism of human anodal tear protein. Biochem Genet 14, 225–235 (1976). https://doi.org/10.1007/BF00484762
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DOI: https://doi.org/10.1007/BF00484762