Abstract
Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5–2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent or in shortage in the incubation medium.
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Zagris, N. Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system. Biochem Genet 15, 825–832 (1977). https://doi.org/10.1007/BF00483979
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DOI: https://doi.org/10.1007/BF00483979