Abstract
A close correlation was observed between body weight and length of the survival time of mice inoculated intravenously (i.v.) with Cryptococcus neoformans (p<0.001). An endotoxic substance of C. neoformans (Cr-ET) increased the susceptibility of mice to i.v. infection of C. neoformans only when more than 50 μg of Cr-ET was injected i.v. 24 hours before infection. Intraperitoneal (i.p.) administration of dimethyl sulfoxide which is found to enhance bacterial infection did not enhance death rate of mice infected i.p. with C. neoformans.
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References
Bennet, J. E. & H. F. Hasenclever. 1965. Cryptococcus neoformans polysaccharide: Studies of serologic properties and role in infection. J. Immunol. 94: 916–920.
Bulmer, G. S., M. D. Sans & C. M. Gunn. 1967. Cryptococcus neoformans. I. Nonencapsulated mutant. J. Bacter. 94: 1475–1479.
Bulmer, G. S. & M. D. Sans. 1967. Cryptococcus neoformans. II. Phagocytosis by human leucocytes. J. Bact. 94: 1480–1483.
Bulmer, G. S. & M. D. Sans. 1968. Cryptococcus neoformans. III. Inhibition of phagocytosis. J. Bact. 95: 5–8.
Cluff, L. E. 1970. Review; Effects of endotoxin of susceptibility to infections. J. Infect. Dis. 122: 205–215.
Gadebusch, H. H. 1958. Active immunization against Cryptococcus neoformans. J. Infect. Dis. 102: 219–226.
Iwata, K. & T. Nagai. 1971. Studies on the methodology for experimental fungus infections. I. Relationship between inoculum size and virulence. Jap. J. Med. Mycol. 12: 13–17.
Kato, N., F. Ohta, T. Kobayashi & I. Nakashima. 1973. Effect of Dimethyl sulfoxide on natural resistance to bacterial infections in mice. Jap. J. Microbiol. (in press).
Kobayashi, H. 1969. Effect of Escherichia coli and its endotoxin on the infection of mice with Cryptococcus neoformans alone. Jap. J. Med. Mycol. 10: 298–304.
Kobayashi, H. 1969. Effect of Escherichia coli and its endotoxin on the resistance of mice to experimental cryptococcal infection. Jap. J. Microbiol., 13: 223–224.
Kobayashi, T., I. Nakashima & N. Kato. 1973. Endotoxic substance of Cryptococcus neoformans. Mycopath. Mycol. Appl. (in press).
Kunze, M. & H. J. Klein. 1971. Der Einfluss von Dimethylsulfoxide auf die experimentelle intraperitoneale Staphylokokken-Infektion der Swiss-Albino-Maus. Zentbl. Bakt., I. Abt. Orig. 216: 175–184.
Mulholland, J. H. & L. E. Cluff. 1964. The effect of endotoxin upon susceptibility to infection: The role of the granulocyte. In Bacterial Endotoxins, Landy, M. & W. Braun. Eds. pp. 211–229. Rutgers Univ. Press, New Brunswick, N.J.
Rowley. 1956. Rapidly induced changes in the level of non-specific immunity in laboratory animals. Brit. J. Exp. Pathol. 37: 223–234.
Siegel, S. 1956. Nonparametric Stastics for the Behavioral Science, pp. 111–117, McGraw-Hill Book Co., New York.
Tacker, J. R., F. Farhi & G. S. Bulmer. 1972. Intracellular fate of Cryptococcus neoformans. Inf. Imm. 6: 162–167.
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Kobayashi, T., Kato, O., Nakashima, I. et al. Factors affecting experimental infection with Cryptococcus neoformans in mice with special reference to an endotoxic substance of C. neoformans. Mycopathologia 55, 17–22 (1975). https://doi.org/10.1007/BF00467085
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DOI: https://doi.org/10.1007/BF00467085