Abstract
A chemical method for lipid extraction has been applied to Candida albicans blastospores previously to their examination by transmission electron microscopy. The results led to the concept of a superficial location of lipids bounded to the peptidopolysaccharidic matrix of the cell wall. This lipid extraction also allowed us to describe cell wall microfibrillar structures. Two types of microfibrills have been particularly identified: (1) microfibrils of approximately 50 Å in diameter, involved in a network of the cell wall intermediate layers and supposed to correspond to ß 1–3 glucans; (2) fibrillar structures of 120 Å in diameter, similar to chitin microfibrils, observed in the bud scar septum.
Résumé
Une méthode d'extraction physico-chimique des lipides a été appliquée à des blastospores entières de C. albicans, préalablement à leur étude en microscopie électronique à transmission. Les résultats permettent de supposer une localisation très superficielle des lipides liés à d'autres composants, en particulier peptidopolysaccharidiques, ce qui est en accord avec les données de la littérature. La méthode à également permis l'observation de structures microfibrillaires pariétales. Il s'agit surtout, d'une part, de fibrilles d'environ 50 Å de diamètre, situées dans les couches moyennes, également observables, après mise en oeuvre d'une technique d'extraction de polysaccharides sur cellules entières, et qui ont été assimilées à des fibrilles de ß 1–3 glucanes; d'autre part de fibrilles de 120 À de diamètre, situées au centre du septum de la cicatrice de bourgeonnement et qui ont été assimilées à des fibrilles de chitine.
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Poulain, D., Tronchin, G. & Vernes, A. Application d'une méthode d'extraction des lipides aux parois des blastospores de Candida albicans . Mycopathologia 92, 141–147 (1985). https://doi.org/10.1007/BF00437625
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DOI: https://doi.org/10.1007/BF00437625