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Effect of nystatin, amphotericin B and amphotericin B methyl ester on Saccharomyces cerevisiae with different lipid composition

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Abstract

Saccharomyces cerevisiae was cultured under anaerobiosis in semi-complete medium to which either palmitoleic or oleic acid was added. Cells were grown at 20 °C or 30 °C. The levels of total lipids, total sterols, and phospholipids were higher in cells grown at 20 °C than at 30 °C. The effects of nystatin (NYS), amphotericin B (AMB), and amphotericin B methyl ester (AME) were evaluated by determining cell viability and liberation of intracellular compounds. The loss of cell viability is higher in the first 30 minutes of incubation with the drugs and is the same regardless of the type of cells obtained. Low molecular weight compounds and ions such as K+ are liberated a few minutes after incubation with the drugs whereas proteins and substances absorbing at 260 nm are liberated later. Phosphate liberation comes after K+ and before compounds of higher molecular weights.

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Authors and Affiliations

  1. Departamento de Microbiologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CP 2486, 30161, Belo Horizonte-MG, Brazil

    Maria Aperecida de Resende

  2. Departamento de Microbiologia, Instituto de Ciências Biológicas da Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, 05508, São Paulo, Brazil

    Flávio Alterhum

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  1. Maria Aperecida de Resende
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  2. Flávio Alterhum
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de Resende, M.A., Alterhum, F. Effect of nystatin, amphotericin B and amphotericin B methyl ester on Saccharomyces cerevisiae with different lipid composition. Mycopathologia 112, 165–172 (1990). https://doi.org/10.1007/BF00436649

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