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Chronic megakaryocytic-granulocytic myelosis — An electron microscopic study including freeze-fracture

II. Granulocytes, erythrocytes, plasma cells and myeloid stroma

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Summary

In 5 patients with chronic megakaryocytic-granulocytic myelosis (CMGM) bone marrow specimens were studied by electron microscopy to investigate possible abnormalities of the granulocytic cell lineage. Thin sections were compared with freeze-fracture replicas to elucidate further aspects of leucocyte cytology. The atypia exhibited in these cells (eosinophils, basophils and neutrophil granulocytes) consisted mostly of a disorganization of granulopoiesis with hyper- and hypogranulation, a pathological increase in the number of nuclear blebs and a maturation asynchrony sometimes leading to Pelger-like cell forms. Moreover, a presumptive stem cell was demonstrated in the erythopoietic and granulocytic cell lines resembling CFU cells. In conclusion, granulopoiesis in CMGM exhibited abnormalities as generally observed in chronic myelogenous leukaemia. When considered with our previous finding of malignancy in megakaryopoiesis, CMGM has to be classified as a myelosis of mixed cellularity.

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Abbreviations

CMGM:

Chronic megakaryocytic-granulocytic myelosis

CGL:

Chronic granulocytic leukaemia

MF:

Osteomyelofibrosis

RER:

Rough surfaced endoplasmic reticulum

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Authors and Affiliations

  1. Pathologisches Institut, Medizinische Hochschule Hannover, Karl-Wiechert-Allee 9, D-3000, Hannover, Federal Republic of Germany

    J. Thiele, A. -Ch. Ballard, A. Georgii & K. F. Vykoupil

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  1. J. Thiele
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  2. A. -Ch. Ballard
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  3. A. Georgii
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  4. K. F. Vykoupil
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Supported by the Deutsche Forschungsgemeinschaft, grant Ge 121/15

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Thiele, J., Ballard, A.C., Georgii, A. et al. Chronic megakaryocytic-granulocytic myelosis — An electron microscopic study including freeze-fracture. Virchows Arch. A Path. Anat. and Histol. 375, 129–146 (1977). https://doi.org/10.1007/BF00428101

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