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Tumour necrosis factor-α levels and the effects of pentoxifylline in cats with feline leukaemia virus infection

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Abstract

Tumour necrosis factor-α (TNF-α) levels were measured in supernatants of macrophage cultures infected in vitro with feline leukaemia virus (FeLV) and in serum, bone marrow plasma, and supernatants of bone marrow mononuclear cell (BMMC) cultures from cats infected with an anemogenic strain of FeLV. TNF-α levels significantly increased (p < 0.05) in the supernatants of in vitro FeLV-infected macrophage cultures compared with uninfected cultures. TNF-α levels in serum and culture supernatants of BMMC from FeLV-infected cats were also significantly increased (p < 0.05) compared with baseline values. Pentoxifylline is a dimethylxanthine derivative that inhibits TNF-α production. Pentoxifylline treatment of FeLV-infected macrophage cultures caused a significant decrease in TNF-α levels. Pentoxifylline given orally to FeLV-infected cats from week 0 to week 5 post-inoculation did not cause significant inhibition of TNF-α levels. Although a trend toward higher numbers of erythroid and fibroblast progenitors was observed in pentoxifylline-treated cats, the changes were not significantly different. The persistent increase in TNF-α levels in serum and culture supernatants of BMMC from FeLV-infected cats suggests that TNF-α may be important in the pathogenesis of hematopoietic suppression.

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Authors and Affiliations

  1. Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA

    K. N. M. Khan, G. J. Kociba, M. L. Wellman & J. A. Reiter

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  1. K. N. M. Khan
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  2. G. J. Kociba
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  3. M. L. Wellman
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  4. J. A. Reiter
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Khan, K.N.M., Kociba, G.J., Wellman, M.L. et al. Tumour necrosis factor-α levels and the effects of pentoxifylline in cats with feline leukaemia virus infection. Comparative Haematology International 2, 133–139 (1992). https://doi.org/10.1007/BF00426166

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