Summary
-
1.
The order of magnitude of the incorporation of uridine into RNA within 15 min is comparable in normal synchronous, N-deficient, and recovering cultures of Chlorella pyrenoidosa.
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2.
The kinetics of the incorporation of uridine into RNA show the kind of curves to be expected from the theory for systems with several products or several compartments.
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3.
When the cultures are treated with rifampin or chloramphenicol, uridine incorporation is much more inhibited in normal cells than in N-deficient cells, while treatment with cycloheximide has only slight effects in both cases.
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4.
In N-deficient and recovering cultures, 3H-uridine activity can be shifted by chase treatment from ribosomal precursors to 80S ribosomes (in contrast to normal cultures where it can be shifted to 70S ribosomes).
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5.
Particles isolated from a membrane-rich cell fraction by DOC treatment are labelled much more quickly by 3H-uridine in N-deficient than in normal cells.
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6.
Under conditions of nitrogen deficiency, uridine incorporation into chloroplastic ribosomal particles is not so greatly preferred as it is in Chlorella under normal conditions. Consequences of these results are discussed which concern the control of ribosome synthesis in Chlorella.
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Abbreviations
- bis-MSB:
-
bis-(O-methylstyryl)benzene
- DOC:
-
sodium deoxycholate
- ER:
-
endoplasmic reticulum
- PPO:
-
2,5-diphenyl oxazol
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Sections of this paper are part of a doctor thesis presented to the Mathematisch-Naturwissenschaftliche Fakultät der Universität Göttingen.
Postdoctoral fellow of the Deutsche Forschungsgemeinschaft.
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Ssymank, V. Influence of nitrogen deficiency on uridine incorporation into ribosomes in the green alga Chlorella . Archiv. Mikrobiol. 82, 311–324 (1972). https://doi.org/10.1007/BF00424935
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DOI: https://doi.org/10.1007/BF00424935