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Chromosomal proteins of Physarum polycephalum with preferential affinity for the sequence, poly d(A-T). poly d(A-T)

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Abstract

We have identified two novel chromosomal proteins from Physarum polycephalum using a protein blotting DNA-binding assay. A fraction of these proteins was readily released from nuclei by solutions of moderate ionic strength (0.15 N–0.35 M NaCl) or mild nuclease treatment and appear associated with chromatin that is nucleosome-free. A significant proportion of these proteins, however, was not released from nuclei by solutions of high ionic strength (1.6 M NaCl) or treatment with excess nuclease. These results suggest that these chromosomal proteins are distributed between transcriptionally-competent and inert domains of chromatin. Both proteins preferentially and tenaciously bound duplex DNA, especially to the alternating B-DNA conformation displayed by the synthetic sequence, poly d(A-T).poly d(A-T).

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Abbreviations

SDS:

sodium dodecyl sulfate

PMSF:

phenylmethylsulfonyl fluoride

bp:

base pairs

kD:

kilodaltons

HMG:

high-mobility-group

PAGE:

polyacrylamide gel electrophoresis

DNase I:

deoxyribonuclease I

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Authors and Affiliations

  1. Department of Biology, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada

    Katharine A. Edens Magor & Jonathan M. Wright

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  1. Katharine A. Edens Magor
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  2. Jonathan M. Wright
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Edens Magor, K.A., Wright, J.M. Chromosomal proteins of Physarum polycephalum with preferential affinity for the sequence, poly d(A-T). poly d(A-T). Mol Biol Rep 16, 105–115 (1992). https://doi.org/10.1007/BF00419756

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  • DOI: https://doi.org/10.1007/BF00419756

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