Abstract
By means of the described technique lens epithelia were cultured in 40 subcultures; at the same time, both the morphological aging processes and oxygen consumption of the cells, were observed using the Warburg respirometer. To date comparable studies are not available. Morphologically, cell enlargement took place in conjunction with signs of degeneration. Oxygen consumption of 2 μl per hour per 105 cells ±0.8 remained at a constant level without any significant diurnal rhythm up to subculture 39 and then suddenly fell to about one-fourth of the initial value. As the cells died of an unknown cause, it was concluded that the aerobic metabolism of lens epithelia seemed to be linked to survival without being age related.
Zusammenfassung
Mit Hilfe der beschriebenen Technik gelang es, Linsenepithelien über 40 Passagen zu züchten; zugleich fanden morphologische Alterungsveränderungen und Sauerstoffverbrauch der Zellen im Warburg-Apparat Beachtung. Vergleichbare Untersuchungen sind derzeit nicht bekannt. Morphologisch überwogen Alterationen wie Vergrößerung des Zelleibes neben bekannten Degenerationszeichen. Der Sauerstoffverbrauch von 2 μl pro Stunde pro 105 Zellen zeigte sich in konstanter Höhe bis zur 39. Passage und sank dann abrupt auf etwa ein Viertel. Nachdem die Zellen ohne ersichtlichen Grund danach plötzlich starben, liegt die Folgerung nahe, daß der aerobe Stoffwechsel der Linsenepithelien unabhängig von ihrem Alter und ohne sich signifikant abzeichnenden Tagesrhythmus mit ihrem Überleben verbunden erscheint.
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Herrn Prof. Dr. E. Schreck, dem damaligen Direktor der Klinik, in Dankbarkeit zum 70. Geburtstag
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Mayer, U.M., Sames, K. Sauerstoffverbrauch und Alterung in Langzeitkulturen von Linsenepithel. Albrecht von Graefes Arch. Klin. Ophthalmol. 217, 117–124 (1981). https://doi.org/10.1007/BF00418986
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DOI: https://doi.org/10.1007/BF00418986